Cloning and High-Level Expression of Scorpion Toxin Bmkita1 in Escherichia Coli and Insect Cells

Title: Cloning and High-Level Expression of Scorpion Toxin Bmkita1 in Escherichia Coli and Insect Cells

Volume: 9 Issue: 5

Author(s): Zhen Liu, Guan-Zhen Yang, Cheng-Wu Chi and Xiang-Fu Wu

Affiliation:

Keywords: BmK ITa1, E. coli, baculovirus expression, Tn-5B1-4 cell, GenBank, Accession Number AY090782

Abstract: BmK ITa1 cDNA was cloned and highly expressed in E. coli and insect cell. SDS-PAGE and western blot analysis revealed that subunit molecular weight of expression products is about 40 kDa and 10 kDa respectively. The expression product purified by a Ni2+-IDA-sepharose 6B column was toxic for insect, which indicated that it was biologically activity. Furthermore, Quantitative estimation show that the biological activity of recombinant BmK ITa1 from Tn cells was more powerful than from E. coli.

Cite this article as:

Liu Zhen, Yang Guan-Zhen, Chi Cheng-Wu and Wu Xiang-Fu, Cloning and High-Level Expression of Scorpion Toxin Bmkita1 in Escherichia Coli and Insect Cells, Protein & Peptide Letters 2002; 9 (5) . https://dx.doi.org/10.2174/0929866023408472