Conformational Analysis of Sea Cucumber (Caudina Arenicola) C Globin 94-97 Fragment, Pro-Glu-Leu-Leu

Title: Conformational Analysis of Sea Cucumber (Caudina Arenicola) C Globin 94-97 Fragment, Pro-Glu-Leu-Leu

Volume: 9 Issue: 5

Author(s): P.N. Sunil Kumar, K. S. Devaky, C. Sadasivan and M. Haridas

Affiliation:

Keywords: Conformational analysis, sea cucumber, C globin, two-dimensional NMR

Abstract: The tetrapeptide Pro-Glu-Leu-Leu forms the 94-97 fragment of C globin in sea cucumber. 2% Butanediol dimethacrylate-cross linked polystyrene (2% BDDMA-PS), which had been optimized, was used for the synthesis of the tetrapeptide Pro-Glu-Leu-Leu. The peptide was synthesized by using Boc-amino acid strategy. The peptide purity was checked by RP-HPLC and the peptide was characterized by ¹H NMR spectroscopy and amino acid analysis. Conformation of the peptide was studied by 1D- and 2D- homonuclear ¹H NMR, in DMSO-d6 at 300K. The conformation of the synthetic tetrapeptide (extended backbone conformation) is not in agreement with that in C globin.

Cite this article as:

Kumar Sunil P.N., Devaky S. K., Sadasivan C. and Haridas M., Conformational Analysis of Sea Cucumber (Caudina Arenicola) C Globin 94-97 Fragment, Pro-Glu-Leu-Leu, Protein & Peptide Letters 2002; 9 (5) . https://dx.doi.org/10.2174/0929866023408526