Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase

Matsujiro      Ishibashi; Tsutomu      Arakawa; Masao      Tokunaga

doi:10.2174/0929866033478546

Abstract

Previously we reported that halobacterial nucleoside diphosphate kinase can be refolded in the presence of concentrated trimethylamine N-oxide (TMAO) as well as NaCl, indicating that enhancement of compact structure formation by TMAO is sufficient for folding. Here we showed that the refolding effect of MgCl2 is maximal at 1 M and declines to zero at 2 M, indicating that charge shielding effect of MgCl2 is offset by its salting-in effect.

Keywords: halophilic, nucleoside diphosphate kinase, refolding, charge shielding, salting-in

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Protein & Peptide Letters

Title: Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase

Volume: 10 Issue: 6

Author(s): Matsujiro Ishibashi, Tsutomu Arakawa and Masao Tokunaga

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Keywords: halophilic, nucleoside diphosphate kinase, refolding, charge shielding, salting-in

Abstract: Previously we reported that halobacterial nucleoside diphosphate kinase can be refolded in the presence of concentrated trimethylamine N-oxide (TMAO) as well as NaCl, indicating that enhancement of compact structure formation by TMAO is sufficient for folding. Here we showed that the refolding effect of MgCl2 is maximal at 1 M and declines to zero at 2 M, indicating that charge shielding effect of MgCl2 is offset by its salting-in effect.

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Ishibashi Matsujiro, Arakawa Tsutomu and Tokunaga Masao, Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase, Protein & Peptide Letters 2003; 10 (6) . https://dx.doi.org/10.2174/0929866033478546