Purification and Crystallization of a Lysr-Type Transcriptional Regulator Cbnr from Ralstonia Eutropha Nh9

Title: Purification and Crystallization of a Lysr-Type Transcriptional Regulator Cbnr from Ralstonia Eutropha Nh9

Volume: 10 Issue: 3

Author(s): Shin Muraoka, Rumi Okumura, Yoshitaka Uragami, Takamasa Nonaka, Naoto Ogawa, Kiyotaka Miyashita and Toshiya Senda

Affiliation:

Keywords: cbnr, lysr-type transcriptional regulator, ralstonia eutropha nh9, vapor-diffusion, orthorhombic system, lttrs

Abstract: CbnR, a LysR-type transcriptional regulator from Ralstonia eutropha NH9, has been crystallized by the vapor-diffusion method. It is intriguing to note that the different mixing ratios between the protein and reservoir solutions resulted in the different crystal forms. These crystals have the symmetry of the orthorhombic system with space groups P21212 and P2 1 2 1 2 1.

Cite this article as:

Muraoka Shin, Okumura Rumi, Uragami Yoshitaka, Nonaka Takamasa, Ogawa Naoto, Miyashita Kiyotaka and Senda Toshiya, Purification and Crystallization of a Lysr-Type Transcriptional Regulator Cbnr from Ralstonia Eutropha Nh9, Protein & Peptide Letters 2003; 10 (3) . https://dx.doi.org/10.2174/0929866033478942