Oxidation of Goat Hepatic Galectin-1 Induces Change in Secondary Structure

Abhay   H.   Pande; Rajesh   K. Gupta   Sumati; Krishnan      Hajela

doi:10.2174/0929866033478960

Abstract

Galectin-1 requires a reducing environment for its lectin activity and the carbohydrate binding function is destroyed in oxidizing condition. In this report we provide direct evidence that the oxidation of goat hepatic galectin-1 perturbs its carbohydrate recognition domain and this could be due to changes in secondary structure of goat hepatic galectin-1. Conformational changes in goat hepatic galectin-1 due to oxidation were investigated by absorption, fluorescence and circular dichroism measurements.

Keywords: galectin-1, oxidation, conformational change

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Protein & Peptide Letters

Title: Oxidation of Goat Hepatic Galectin-1 Induces Change in Secondary Structure

Volume: 10 Issue: 3

Author(s): Abhay H. Pande, Rajesh K. Gupta Sumati and Krishnan Hajela

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Keywords: galectin-1, oxidation, conformational change

Abstract: Galectin-1 requires a reducing environment for its lectin activity and the carbohydrate binding function is destroyed in oxidizing condition. In this report we provide direct evidence that the oxidation of goat hepatic galectin-1 perturbs its carbohydrate recognition domain and this could be due to changes in secondary structure of goat hepatic galectin-1. Conformational changes in goat hepatic galectin-1 due to oxidation were investigated by absorption, fluorescence and circular dichroism measurements.

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Pande H. Abhay, Sumati K. Gupta Rajesh and Hajela Krishnan, Oxidation of Goat Hepatic Galectin-1 Induces Change in Secondary Structure, Protein & Peptide Letters 2003; 10 (3) . https://dx.doi.org/10.2174/0929866033478960