Crystallization And Preliminary X-Ray Analysis Of Class Ii Fructose-1,6-Bisphosphate Aldolase From Thermus Caldophilus

Title: Crystallization And Preliminary X-Ray Analysis Of Class Ii Fructose-1,6-Bisphosphate Aldolase From Thermus Caldophilus

Volume: 10 Issue: 5

Author(s): Jun Hyuck Lee, Young Jun Im, Seong-Hwan Rho, Seong Ho Park, Mun-Kyoung Kim, Su Jin Cho, Tae-Yeon Kim, Jin Hwan Oh, Hyun-Jae Shin, Dae-Sil Lee and Soo Hyun Eom

Affiliation:

Keywords: fructose, bisphosphate aldolase, dihydroxyaceton phosphate, glyceraldehydes-3-phosphate, thermus caldophilus

Abstract: In this study, we have crystallized class II fructose-1,6-bisphosphate aldolase (FBA) from Thermus caldophilus (Tca). Purified Tca FBA is a tetrameric enzyme of 305 residues, which crystallizes in the space group P212121 (cell dimensions a = 98.9, b = 113.1, c = 115.7 Å), with four molecules in the asymmetric unit. A complete diffraction data set was obtained from orthorhombic crystals at resolution of 2.2 Å.

Cite this article as:

Lee Hyuck Jun, Im Jun Young, Rho Seong-Hwan, Park Ho Seong, Kim Mun-Kyoung, Cho Jin Su, Kim Tae-Yeon, Oh Hwan Jin, Shin Hyun-Jae, Lee Dae-Sil and Eom Hyun Soo, Crystallization And Preliminary X-Ray Analysis Of Class Ii Fructose-1,6-Bisphosphate Aldolase From Thermus Caldophilus, Protein & Peptide Letters 2003; 10 (5) . https://dx.doi.org/10.2174/0929866033478735