Direct Screening Of Libraries Of Yeast Clones For α-Amylase Activity On Raw Starch Hydrolysis

Title: Direct Screening Of Libraries Of Yeast Clones For α-Amylase Activity On Raw Starch Hydrolysis

Volume: 10 Issue: 5

Author(s): Dominic W.S. Wong, Sarah B. Batt, Charles C. Lee and George H. Robertson

Affiliation:

Keywords: amy1, barley seed amylase, amylase, starch hydrolysis, yeast library, high-throughput assay

Abstract: High-throughput screening for high-activity barley a-amylase mutants expressed in Saccharomyces cerevisiae is hampered by the interference of reducing agents, particularly the glucose used in yeast growth media. The present investigation employed colorimetric and chemiluminescent detection systems that enable direct and rapid screening of activities on raw starch substrate. Active clones could be separated into two groups, based on high total activity or high specific activity.

Cite this article as:

Wong W.S. Dominic, Batt B. Sarah, Lee C. Charles and Robertson H. George, Direct Screening Of Libraries Of Yeast Clones For α-Amylase Activity On Raw Starch Hydrolysis, Protein & Peptide Letters 2003; 10 (5) . https://dx.doi.org/10.2174/0929866033478744