Crystallization and Preliminary X-ray Analysis of Plant Class I Chitinase from Rice

Title: Crystallization and Preliminary X-ray Analysis of Plant Class I Chitinase from Rice

Volume: 11 Issue: 4

Author(s): Yuichiro Kezuka, Keiji Kitazaki, Yoshikane Itoh, Jun Watanabe, Osamu Takaha, Takeshi Watanabe, Yoko Nishizawa and Takamasa Nonaka

Affiliation:

Keywords: class I chitinase, rice (oryza sativa l. japonica), crystallization, preliminary x-ray analysis, protease inhibitor complex, specificity, elastase

Abstract: We report here on crystallization and preliminary X-ray analysis of plant class I chitinase from rice (OsChia1b). Similar single crystals of full-length OsChia1b were obtained under two independent conditions. The crystals grown under these conditions diffracted up to 2.1 and 2.5 Å resolution, respectively, at a synchrotron beamline, and were found to belong to the tetragonal space group P43212.

Cite this article as:

Kezuka Yuichiro, Kitazaki Keiji, Itoh Yoshikane, Watanabe Jun, Takaha Osamu, Watanabe Takeshi, Nishizawa Yoko and Nonaka Takamasa, Crystallization and Preliminary X-ray Analysis of Plant Class I Chitinase from Rice, Protein & Peptide Letters 2004; 11 (4) . https://dx.doi.org/10.2174/0929866043406968