Volumetric Behavior of the Molten Globule State of Canine Milk Lysozyme

Masahiro      Watanabe; Yoshihiro      Kobashigawa; Tomoyasu      Aizawa; Makoto      Demura; Katsutoshi      Nitta

doi:10.2174/0929866043406832

Abstract

The effect of pressure on the unfolding of the molten globule (MG) state of canine milk lysozyme (CML) was examined using ultraviolet spectroscopy. The volume changes of the MG-unfolded-state transition were observed at pH 2.0 and around 20 to 60°C, but no volume change has been found for bovine α-lactalbumin, which is homologous to CML. Our results suggest that the MG state of CML possesses a tightly packed hydrophobic core.

Keywords: canine milk lysozyme, olding, hydration

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Protein & Peptide Letters

Title: Volumetric Behavior of the Molten Globule State of Canine Milk Lysozyme

Volume: 11 Issue: 4

Author(s): Masahiro Watanabe, Yoshihiro Kobashigawa, Tomoyasu Aizawa, Makoto Demura and Katsutoshi Nitta

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Keywords: canine milk lysozyme, olding, hydration

Abstract: The effect of pressure on the unfolding of the molten globule (MG) state of canine milk lysozyme (CML) was examined using ultraviolet spectroscopy. The volume changes of the MG-unfolded-state transition were observed at pH 2.0 and around 20 to 60°C, but no volume change has been found for bovine α-lactalbumin, which is homologous to CML. Our results suggest that the MG state of CML possesses a tightly packed hydrophobic core.

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Watanabe Masahiro, Kobashigawa Yoshihiro, Aizawa Tomoyasu, Demura Makoto and Nitta Katsutoshi, Volumetric Behavior of the Molten Globule State of Canine Milk Lysozyme, Protein & Peptide Letters 2004; 11 (4) . https://dx.doi.org/10.2174/0929866043406832