Crystallization and Preliminary X-Ray Crystallographic Analysis of Plasmodium Falciparum S-Adenosyl-L-Homocysteine Hydrolase

Title: Crystallization and Preliminary X-Ray Crystallographic Analysis of Plasmodium Falciparum S-Adenosyl-L-Homocysteine Hydrolase

Volume: 11 Issue: 2

Author(s): Nobutada Tanaka, Yoshio Kusakabe, Katsura Shiraiwa, Yasumitsu Sakamoto, Masayuki Nakanishi, Yukio Kitade and Kazuo T. Nakamura

Affiliation:

Keywords: Plasmodium, S-adenosyl-L-homocysteine, hydrolase, orthorhombic

Abstract: S-adenosyl-L-homocysteine hydrolase from a malaria parasite Plasmodium falciparum (PfSAHH) has been crystallized by the vapor diffusion method. The crystals belong to an orthorhombic space group P212121 with the cell dimensions of a = 76.66 Å, b = 86.31 Å, and c = 335.6 Å. There are four subunits (one tetramer) per asymmetric unit. X-ray diffraction data have been collected up to 2.8 Å resolution. Selfrotation function studies suggest that the tetrameric PfSAHH molecule has the 222 point group symmetry.

Cite this article as:

Tanaka Nobutada, Kusakabe Yoshio, Shiraiwa Katsura, Sakamoto Yasumitsu, Nakanishi Masayuki, Kitade Yukio and Nakamura T. Kazuo, Crystallization and Preliminary X-Ray Crystallographic Analysis of Plasmodium Falciparum S-Adenosyl-L-Homocysteine Hydrolase, Protein & Peptide Letters 2004; 11 (2) . https://dx.doi.org/10.2174/0929866043478248