Fluorescent Modification Of N-Terminal Amino Group In Peptides For Complete Sequence Analysis Using Psd Method In Maldi-Tof-Ms

Title: Fluorescent Modification Of N-Terminal Amino Group In Peptides For Complete Sequence Analysis Using Psd Method In Maldi-Tof-Ms

Volume: 11 Issue: 1

Author(s): Masatoshi Nakagawa and Hiroshi Nakanishi

Affiliation:

Keywords: maldi-tof-ms, psd method, peptide sequence, fluorescently modification, nucleoprotein in influenza virus

Abstract: In the sequence analyses of peptides from nucleoprotein in influenza virus, it was very difficult to obtain the sufficient numbers of fragment ions using the post-source decay (PSD) method in MALDITOF- MS. Fluorescent modification of the N-terminal amino group in the target peptides was introduced for the PSD measurement. The fluorescently-labelled peptides gave sufficient fragment ions in the PSD spectrum, which leads to the complete sequence analysis of peptide.

Cite this article as:

Nakagawa Masatoshi and Nakanishi Hiroshi, Fluorescent Modification Of N-Terminal Amino Group In Peptides For Complete Sequence Analysis Using Psd Method In Maldi-Tof-Ms, Protein & Peptide Letters 2004; 11 (1) . https://dx.doi.org/10.2174/0929866043478464