Expression, Purification And Characterization Of Recombinant Mouse Pc6b From Baculovirus-Infected Insect Cells

Lie      Wang; Guanzhen      Yang; Xiangfu      Wu

doi:10.2174/0929866043478509

Abstract

we constructed a mouse PC6B truncated mutant and introduced a tag of 6 consecutive histidines at its carboxyl terminus for simple purification. Using the baculovirus expression system and standard enzymatic assay, we obtained recombinant mouse PC6B protein and with enzymatic activity.

Keywords: btmd-mpc6b, insect cells

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Protein & Peptide Letters

Title: Expression, Purification And Characterization Of Recombinant Mouse Pc6b From Baculovirus-Infected Insect Cells

Volume: 11 Issue: 1

Author(s): Lie Wang, Guanzhen Yang and Xiangfu Wu

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Keywords: btmd-mpc6b, insect cells

Abstract: we constructed a mouse PC6B truncated mutant and introduced a tag of 6 consecutive histidines at its carboxyl terminus for simple purification. Using the baculovirus expression system and standard enzymatic assay, we obtained recombinant mouse PC6B protein and with enzymatic activity.

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Wang Lie, Yang Guanzhen and Wu Xiangfu, Expression, Purification And Characterization Of Recombinant Mouse Pc6b From Baculovirus-Infected Insect Cells, Protein & Peptide Letters 2004; 11 (1) . https://dx.doi.org/10.2174/0929866043478509