Comparison of Expression of Monomeric and Multimeric Adenoregulin Genes in Escherichia coli and Pichia pastorias

Title: Comparison of Expression of Monomeric and Multimeric Adenoregulin Genes in Escherichia coli and Pichia pastorias

Volume: 12 Issue: 4

Author(s): Yuxun Zhou, Wei Cao, Jinzhi Wang, Yushu Ma and Dongzhi Wei

Affiliation:

Keywords: antibiotic peptide, adenoregulin, recombinant expression, escherichia coli, pichia patoris

Abstract: Adenoregulin is a 33 amino acid antibiotic peptide who belongs to dermaseptin family which is the first vertebrate family to show lethal effects against filamentous fungi, as well as a broad spectrum of pathogenic microorganisms. Synthetic adenoregulin gene was cloned in 2, 4 and 6 tandem repeats and subcloned in pET32a and pET22b vectors. Recombinant plasmids were transformed into E. coli BL21(DE3), Fusion proteins of Trx-ADR1, Trx- ADR2 and Trx-ADR4 could be expressed after the hosts were induced by IPTG, but the expression level decreased dramatically with the number of tandem repeats increased. ADR1, ADR4 and ADR6 could not be expressed by E. coli without carrier proteins. But for Pichia pastoris GS115, ADR1 and ADR6 in the fermentation broth of the hosts could be detected by ELISA, and the bactericidal activities could also be observed.

Cite this article as:

Zhou Yuxun, Cao Wei, Wang Jinzhi, Ma Yushu and Wei Dongzhi, Comparison of Expression of Monomeric and Multimeric Adenoregulin Genes in Escherichia coli and Pichia pastorias, Protein & Peptide Letters 2005; 12 (4) . https://dx.doi.org/10.2174/0929866053765671