Unraveling Sub-Site Specificities of Peptidic Serine Protease Inhibitors by Substitutional and Structural Analysis

Title: Unraveling Sub-Site Specificities of Peptidic Serine Protease Inhibitors by Substitutional and Structural Analysis

Volume: 12 Issue: 5

Author(s): Wolfgang Hohne and Kai Hilpert

Affiliation:

Keywords: serine protease, elastase, peptidic inhibitor, ovomucoid inhibitor, peptide spot synthesis, substitutional analysis, subsite specificity, x-ray structure

Abstract: The interaction of peptidic inhibitors with serine proteases is investigated using peptide spot synthesis on cellulose sheets. This method permits a highly parallel analysis of subsite specificities and an optimization of peptidic inhibitors towards higher affinity and specificity for a given target protease. Information from crystal structures of corresponding protease/inhibitor complexes may help to explain the observed binding pattern.

Cite this article as:

Hohne Wolfgang and Hilpert Kai, Unraveling Sub-Site Specificities of Peptidic Serine Protease Inhibitors by Substitutional and Structural Analysis, Protein & Peptide Letters 2005; 12 (5) . https://dx.doi.org/10.2174/0929866054395374