Expression, Purification and Partial Characterization of the Krüppel- Associated Box (KRAB) from the Human ZNF2 Protein

Title: Expression, Purification and Partial Characterization of the Krüppel- Associated Box (KRAB) from the Human ZNF2 Protein

Volume: 12 Issue: 6

Author(s): Vincenzo Rivieccio, Riccardo Mannini, Luigi Concilio, Sabato D'Auria, Carlo Pedone and Giovanna Grimaldi

Affiliation:

Keywords: transcriptional repression, krab domain, zinc finger, fusion protein expression

Abstract: The Kruppel-associated box (KRAB) domain is a potent transcription repression bipartite domain, shared by over 400 zinc finger proteins in humans, involved in the regulation of many functions. KRAB domains are both physically and functionally bipartite (A and B modules). The lone KRAB-A and composite KRAB-AB domains from the human ZNF2 protein were over-expressed as recombinant proteins in E. coli, isolated and purified to homogeneity to investigate their structure to function relationship.

Cite this article as:

Rivieccio Vincenzo, Mannini Riccardo, Concilio Luigi, D'Auria Sabato, Pedone Carlo and Grimaldi Giovanna, Expression, Purification and Partial Characterization of the Krüppel- Associated Box (KRAB) from the Human ZNF2 Protein, Protein & Peptide Letters 2005; 12 (6) . https://dx.doi.org/10.2174/0929866054395833