Proteome Analysis of Nelore Bull (Bos taurus indicus) Seminal Plasma

T.   I.   Assumpcao; W.      Fontes; M.   V.   Sousa; C.   A.O.   Ricart

doi:10.2174/0929866054864292

Abstract

The Nelore bull (Bos taurus indicus) seminal plasma proteome was analyzed by MALDI-TOF MS and twodimensional gel electrophoresis. A total of 260 spots were visualized in the 2-DE gel (pI range 3-10) and 13 spots could be identified by peptide mass fingerprinting corresponding to 11 different polypeptides. The results allowed the creation of the first proteomic map of Bos taurus indicus seminal plasma. The roles of the identified proteins in the bull seminal plasma are discussed.

Keywords: proteome, seminal plasma, d-page, bos taurus indicus, mass spectrometry

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Protein & Peptide Letters

Title: Proteome Analysis of Nelore Bull (Bos taurus indicus) Seminal Plasma

Volume: 12 Issue: 8

Author(s): T. I. Assumpcao, W. Fontes, M. V. Sousa and C. A.O. Ricart

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Keywords: proteome, seminal plasma, d-page, bos taurus indicus, mass spectrometry

Abstract: The Nelore bull (Bos taurus indicus) seminal plasma proteome was analyzed by MALDI-TOF MS and twodimensional gel electrophoresis. A total of 260 spots were visualized in the 2-DE gel (pI range 3-10) and 13 spots could be identified by peptide mass fingerprinting corresponding to 11 different polypeptides. The results allowed the creation of the first proteomic map of Bos taurus indicus seminal plasma. The roles of the identified proteins in the bull seminal plasma are discussed.

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Assumpcao I. T., Fontes W., Sousa V. M. and Ricart A.O. C., Proteome Analysis of Nelore Bull (Bos taurus indicus) Seminal Plasma, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864292