Using Siclopps for the Discovery of Novel Antimicrobial Peptides and Their Targets

Lisa   O.   Nilsson; Mostafa      Louassini; Ernesto      Abel-Santos

doi:10.2174/0929866054864247

Abstract

High throughput screening of SICLOPPS libraries afforded six distinct cyclic peptides that inhibit Escherichia coli growth both in liquid and solid media. One of these peptides (LN05) reduced both bacterial growth rate and caused cell aggregation in liquid media. Mutant bacteria immune to LN05 action were obtained at a frequency of 10-7. Overexpression of an E. coli genomic library in the presence of LN05 production resulted in enrichment of a single genomic construct, a fragment of the NarZ gene.

Keywords: cyclic peptides, antimicrobial, functional complementation

« Previous Next »

Rights & Permissions Print Cite

Protein & Peptide Letters

Title: Using Siclopps for the Discovery of Novel Antimicrobial Peptides and Their Targets

Volume: 12 Issue: 8

Author(s): Lisa O. Nilsson, Mostafa Louassini and Ernesto Abel-Santos

Affiliation:

Keywords: cyclic peptides, antimicrobial, functional complementation

Abstract: High throughput screening of SICLOPPS libraries afforded six distinct cyclic peptides that inhibit Escherichia coli growth both in liquid and solid media. One of these peptides (LN05) reduced both bacterial growth rate and caused cell aggregation in liquid media. Mutant bacteria immune to LN05 action were obtained at a frequency of 10-7. Overexpression of an E. coli genomic library in the presence of LN05 production resulted in enrichment of a single genomic construct, a fragment of the NarZ gene.

Export Options

Export File:

RIS (for EndNote, Reference Manager, ProCite)

BibTeX

Text

Content:

Citation Only

Citation and Abstract

About this article

Cite this article as:

Nilsson O. Lisa, Louassini Mostafa and Abel-Santos Ernesto, Using Siclopps for the Discovery of Novel Antimicrobial Peptides and Their Targets, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864247