Expressed Protein Ligation to Obtain Selectively Modified Aldo/Keto Reductases

Michael   P.O.   Richter; Annette   G.   Beck-Sickinger

doi:10.2174/0929866054864210

Abstract

Specific enzyme immobilization has moved into the focus for many applications in biochemical research fields. Expressed Protein Ligation (EPL) has been proven to be ideal to selectively label proteins at single positions. Applying this technique to enzymes of the aldo/keto reductase superfamily provides a new approach to generate native or modified redox enzymes for direct and indirect immobilization.

Keywords: expressed protein ligation (epl), site specific covalent immobilization, akr a, artificial redoxproteins, aldo/keto reductase (akr)

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Protein & Peptide Letters

Title: Expressed Protein Ligation to Obtain Selectively Modified Aldo/Keto Reductases

Volume: 12 Issue: 8

Author(s): Michael P.O. Richter and Annette G. Beck-Sickinger

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Keywords: expressed protein ligation (epl), site specific covalent immobilization, akr a, artificial redoxproteins, aldo/keto reductase (akr)

Abstract: Specific enzyme immobilization has moved into the focus for many applications in biochemical research fields. Expressed Protein Ligation (EPL) has been proven to be ideal to selectively label proteins at single positions. Applying this technique to enzymes of the aldo/keto reductase superfamily provides a new approach to generate native or modified redox enzymes for direct and indirect immobilization.

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Richter P.O. Michael and Beck-Sickinger G. Annette, Expressed Protein Ligation to Obtain Selectively Modified Aldo/Keto Reductases, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864210