Mimicking Reverse Protein Splicing by Three-Segment Tandem Peptide Ligation

James   P.   Tam; Khee   D.   Eom

doi:10.2174/0929866054864238

Abstract

Here we report a bi-directional and interchangeable three-segment peptide ligation of N, M, and C-segments, mimicking the reverse process of protein splicing to form, in tandem, a tripartite NMC-peptide using a synthetic intein, a role served by the M-segment with an N-terminal Ser or Thr and a C-terminal thioester.

Keywords: reverse protein splicing, three-segment tandem ligation, cys ligation, pro ligation, synthetic intein, c-to-n tandem ligation, n-to-c tandem ligation, tripartite nmc-protein

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Protein & Peptide Letters

Title: Mimicking Reverse Protein Splicing by Three-Segment Tandem Peptide Ligation

Volume: 12 Issue: 8

Author(s): James P. Tam and Khee D. Eom

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Keywords: reverse protein splicing, three-segment tandem ligation, cys ligation, pro ligation, synthetic intein, c-to-n tandem ligation, n-to-c tandem ligation, tripartite nmc-protein

Abstract: Here we report a bi-directional and interchangeable three-segment peptide ligation of N, M, and C-segments, mimicking the reverse process of protein splicing to form, in tandem, a tripartite NMC-peptide using a synthetic intein, a role served by the M-segment with an N-terminal Ser or Thr and a C-terminal thioester.

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Tam P. James and Eom D. Khee, Mimicking Reverse Protein Splicing by Three-Segment Tandem Peptide Ligation, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864238