Synthesis of Proteins by Native Chemical Ligation Using Fmoc-Based Chemistry

Julio   A.   Camarero; Alexander   R.   Mitchell

doi:10.2174/0929866054864166

Abstract

C-terminal peptide α-thioesters are valuable intermediates in the synthesis/semisynthesis of proteins by native chemical ligation. They are prepared either by solid-phase peptide synthesis (SPPS) or biosynthetically by protein splicing techniques. The present paper reviews the different methods available for the chemical synthesis of peptide α-thioesters using Fmoc-based SPPS.

Keywords: chemical protein synthesis, native chemical ligation, peptide thioester, hydrazine linker, protein engineering, fmoc-chemistry

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Protein & Peptide Letters

Title: Synthesis of Proteins by Native Chemical Ligation Using Fmoc-Based Chemistry

Volume: 12 Issue: 8

Author(s): Julio A. Camarero and Alexander R. Mitchell

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Keywords: chemical protein synthesis, native chemical ligation, peptide thioester, hydrazine linker, protein engineering, fmoc-chemistry

Abstract: C-terminal peptide α-thioesters are valuable intermediates in the synthesis/semisynthesis of proteins by native chemical ligation. They are prepared either by solid-phase peptide synthesis (SPPS) or biosynthetically by protein splicing techniques. The present paper reviews the different methods available for the chemical synthesis of peptide α-thioesters using Fmoc-based SPPS.

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Camarero A. Julio and Mitchell R. Alexander, Synthesis of Proteins by Native Chemical Ligation Using Fmoc-Based Chemistry, Protein & Peptide Letters 2005; 12 (8) . https://dx.doi.org/10.2174/0929866054864166