A Simple Method for Constructing a Tagged Protein

C.      Jo; J.      Kim; S.   A.   Jo

doi:10.2174/0929866054696064

Abstract

We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.

Keywords: gene fusion, recombinant protein, affinity tag, polymerase chain reaction (pcr), small ubiquitin-related modifier (sumo)

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Protein & Peptide Letters

Title: A Simple Method for Constructing a Tagged Protein

Volume: 12 Issue: 7

Author(s): C. Jo, J. Kim and S. A. Jo

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Keywords: gene fusion, recombinant protein, affinity tag, polymerase chain reaction (pcr), small ubiquitin-related modifier (sumo)

Abstract: We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.

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Jo C., Kim J. and Jo A. S., A Simple Method for Constructing a Tagged Protein, Protein & Peptide Letters 2005; 12 (7) . https://dx.doi.org/10.2174/0929866054696064