Crystallization and Preliminary X-Ray Studies of the Unliganded Wild-Type Bovine Thrombin

Daisuke      Iyaguchi; Min      Yao; Nobuhisa      Watanabe; Isao      Tanaka; Eiko      Toyota

doi:10.2174/092986607782110248

Abstract

Wild type of bovine thrombin has been crystallized in a ligand-free form by the hanging drop vapor diffusion method with polyethylene glycol 4000 and 2-propanol. The crystals belong to space group P43212 with unit cell parameters of a = b = 87.7 Å, c = 195.9 Å. X-ray diffraction data were collected to 2.8 Å resolution.

Keywords: Thrombin, blood coagulation system, crystallization

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Protein & Peptide Letters

Title: Crystallization and Preliminary X-Ray Studies of the Unliganded Wild-Type Bovine Thrombin

Volume: 14 Issue: 9

Author(s): Daisuke Iyaguchi, Min Yao, Nobuhisa Watanabe, Isao Tanaka and Eiko Toyota

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Keywords: Thrombin, blood coagulation system, crystallization

Abstract: Wild type of bovine thrombin has been crystallized in a ligand-free form by the hanging drop vapor diffusion method with polyethylene glycol 4000 and 2-propanol. The crystals belong to space group P43212 with unit cell parameters of a = b = 87.7 Å, c = 195.9 Å. X-ray diffraction data were collected to 2.8 Å resolution.

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Iyaguchi Daisuke, Yao Min, Watanabe Nobuhisa, Tanaka Isao and Toyota Eiko, Crystallization and Preliminary X-Ray Studies of the Unliganded Wild-Type Bovine Thrombin, Protein & Peptide Letters 2007; 14 (9) . https://dx.doi.org/10.2174/092986607782110248