Preparation, Crystallization and Preliminary X-Ray Analysis of Threonine Synthase from Streptococcus mutans

Title: Preparation, Crystallization and Preliminary X-Ray Analysis of Threonine Synthase from Streptococcus mutans

Volume: 14 Issue: 8

Author(s): De-Wei Tang, Lan-Fen Li, Ya-Mei Yu, Xiang-Yu Liu, Xiao-Dong Su, Xiaojun Zhao and Yu-He Liang

Affiliation:

Keywords: pyridoxal 5-phosphate, X-ray diffraction data, polymerase chain reaction amplification, Protein Purification, Threonine synthase

Abstract: The thrC gene of Streptococcus mutans encodes threonine synthase, which is a potential target for drug design. To study the structure and function of the enzyme, the thrC gene was amplified from Streptococcus mutans genomic DNA and cloned into the expression vector pET28α. The protein was expressed in Escherichia coli in soluble form and purified to homogeneity. Crystals suitable for X-ray diffraction were obtained by hanging-drop vapor diffusion method. The crystal diffracted to 2.5 Å and belonged to space group P31 or P32, with unit cell parameters a=b=60.39 Å, c=118.62 Å.

Cite this article as:

De-Wei Tang , Lan-Fen Li , Ya-Mei Yu , Xiang-Yu Liu , Xiao-Dong Su , Xiaojun Zhao and Yu-He Liang , Preparation, Crystallization and Preliminary X-Ray Analysis of Threonine Synthase from Streptococcus mutans, Protein & Peptide Letters 2007; 14 (8) . https://dx.doi.org/10.2174/092986607781483633