Endothelin and Its Receptor Interactions: Role of Extracellular Receptor Domain and Length of Peptide Ligands

Title: Endothelin and Its Receptor Interactions: Role of Extracellular Receptor Domain and Length of Peptide Ligands

Volume: 14 Issue: 8

Author(s): K. Saravanan, G. Hariprasad, O. Jitesh, U. Das, S. Dey, S. Sharma, P. Kaur, T.P. Singh and A. Srinivasan

Affiliation:

Keywords: Recombinant human endothelin B receptors, Pichia expression, synthetic peptides, competitive binding

Abstract: Human endothelin B receptor and its domain-truncated forms were cloned and expressed in Pichia pastoris. Ligand binding studies with expressed proteins were carried out using biotinylated endothelins. Competitive binding and liposome incorporation studies showed that the extracellular region is essential for ligand binding and that longer peptides have higher affinity.

Cite this article as:

K. Saravanan , G. Hariprasad , O. Jitesh , U. Das , S. Dey , S. Sharma , P. Kaur , T.P. Singh and A. Srinivasan , Endothelin and Its Receptor Interactions: Role of Extracellular Receptor Domain and Length of Peptide Ligands, Protein & Peptide Letters 2007; 14 (8) . https://dx.doi.org/10.2174/092986607781483651