Cautionary Tail: The Presence of an N-Terminal Tag on Dynein Light-Chain Roadblock/LC7 Affects Its Interaction with a Functional Partner

Jikui      Song; John   L.   Markley; Jikui Song; John L. Markley

doi:10.2174/092986607780090801

Abstract

As part of structural investigations of components of the molecular motor, dynein, we prepared the light chain, Robl1_mouse, with and without an N-terminal His-tag. We found that the His-tag introduced a spurious binding site for a second protein, IC74. We propose a molecular mechanism for functional interference by the His-tag.

Keywords: Affinity tag, docking, dynein, NMR spectroscopy, protein dynamics, protein-protein interactions

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Protein & Peptide Letters

Title: Cautionary Tail: The Presence of an N-Terminal Tag on Dynein Light-Chain Roadblock/LC7 Affects Its Interaction with a Functional Partner

Volume: 14 Issue: 3

Author(s): Jikui Song, John L. Markley, Jikui Song and John L. Markley

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Keywords: Affinity tag, docking, dynein, NMR spectroscopy, protein dynamics, protein-protein interactions

Abstract: As part of structural investigations of components of the molecular motor, dynein, we prepared the light chain, Robl1_mouse, with and without an N-terminal His-tag. We found that the His-tag introduced a spurious binding site for a second protein, IC74. We propose a molecular mechanism for functional interference by the His-tag.

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Song Jikui, Markley L. John, Jikui Song and John L. Markley , Cautionary Tail: The Presence of an N-Terminal Tag on Dynein Light-Chain Roadblock/LC7 Affects Its Interaction with a Functional Partner, Protein & Peptide Letters 2007; 14 (3) . https://dx.doi.org/10.2174/092986607780090801