Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies

Title: Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies

Volume: 14 Issue: 1

Author(s): Tengjiao Cui, Suzanne Joynt, Victor Morillo, Maria Baez, Zhichun Hua, Xiaotang Wang and Fenfei Leng

Affiliation:

Keywords: The mammalian high mobility group protein A2 (HMGA2), DNA binding protein, isothermal titration calorimetry, differential scanning calorimetry, nuclear magnetic resonance (NMR) Spectroscopy, intrinsically unstructured protein

Abstract: Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.

Cite this article as:

Cui Tengjiao, Joynt Suzanne, Morillo Victor, Baez Maria, Hua Zhichun, Wang Xiaotang and Leng Fenfei, Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies, Protein & Peptide Letters 2007; 14 (1) . https://dx.doi.org/10.2174/092986607779117281