Kinetic and Thermodynamic Properties of an Immobilized Glucoamylase from a Mesophilic Fungus, Arachniotus citrinus

Title: Kinetic and Thermodynamic Properties of an Immobilized Glucoamylase from a Mesophilic Fungus, Arachniotus citrinus

Volume: 13 Issue: 7

Author(s): Raheela Perveen, Muhammad Hamid Rashid, Muhammad Saleem, Ahmad Mukhtar Khalid and Muhammad Ibrahim Rajoka

Affiliation:

Keywords: Glucoamylase, enthalpy, entropy, thermodynamics, Arachniotus citrinus

Abstract: Purified glucoamylase from Arachniotus citrinus was immobilized on polyacrylamide gel with 70% yield of immobilization. The immobilization improved the pH optima, temperature optima, values of Km, Vmax, and activation energy. Irreversible thermal denaturation studies of soluble and immobilized glucoamylase indicated that immobilization decreased the entropy and enthalpy of deactivation by magnitudes and made the immobilized glucoamylase thermodynamically more stable.

Cite this article as:

Perveen Raheela, Hamid Rashid Muhammad, Saleem Muhammad, Mukhtar Khalid Ahmad and Ibrahim Rajoka Muhammad, Kinetic and Thermodynamic Properties of an Immobilized Glucoamylase from a Mesophilic Fungus, Arachniotus citrinus, Protein & Peptide Letters 2006; 13 (7) . https://dx.doi.org/10.2174/092986606777790539