Protein Expression, Crystallization and Preliminary X-Ray Crystallographic Studies on HSCARG from Homo Sapiens

Title: Protein Expression, Crystallization and Preliminary X-Ray Crystallographic Studies on HSCARG from Homo Sapiens

Volume: 13 Issue: 9

Author(s): Xueyu Dai, Xiaocheng Gu, Ming Luo and Xiaofeng Zheng

Affiliation:

Keywords: Homo sapiens, HSCARG, protein crystallography

Abstract: Human HSCARG has been annotated as a possible cancer related protein. Amino acid homology, although at a low percentage, suggested that HSCARG contains NmrA domain and might be a member of short chain dehydrogenase reductase superfamily. In order to investigate its structure and function, HSCARG gene has been successfully expressed and purified in E. coli. HSCARG was crystallized and diffracted to a resolution of 2.4 Å on Mar225 CCD Detector at SER-CAT 22BM synchrotron source. The crystals belong to F23 space group, with unit cell parameters a=b=c=223.30Å, α=β=γ=90°. There are two molecules per asymmetry unit.

Cite this article as:

Dai Xueyu, Gu Xiaocheng, Luo Ming and Zheng Xiaofeng, Protein Expression, Crystallization and Preliminary X-Ray Crystallographic Studies on HSCARG from Homo Sapiens, Protein & Peptide Letters 2006; 13 (9) . https://dx.doi.org/10.2174/092986606778256135