Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of the Human Intracellular Chloride Channel Protein CLIC4

Title: Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of the Human Intracellular Chloride Channel Protein CLIC4

Volume: 13 Issue: 6

Author(s): De-Feng Li, Yun-Feng Li, Qiu-Hua Huang, Ying Zhang and Da-Cheng Wang

Affiliation:

Keywords: CLIC4, human chloride intracellular channel protein, purification, crystallization, preliminary crystallographic studies

Abstract: The human chloride intracellular channel protein CLIC4 has been crystallized by the hanging-drop vapourdiffusion technique using trisodium citrate as the precipitant. The best crystals were obtained by the microseeding method. The crystals diffracted to 2.2 Å resolution and were found to belong to space group P121, with unit-cell parameters a = 73.19, b =86.05, c = 73.38 Å, β = 112.99° and three molecule per asymmetric unit.

Cite this article as:

Li De-Feng, Li Yun-Feng, Huang Qiu-Hua, Zhang Ying and Wang Da-Cheng, Expression, Purification, Crystallization and Preliminary Crystallographic Analysis of the Human Intracellular Chloride Channel Protein CLIC4, Protein & Peptide Letters 2006; 13 (6) . https://dx.doi.org/10.2174/092986606777145742