Crystallization and Preliminary X-Ray Crystallographic Analysis of Yeast Tyrosyl-tRNA Synthetase Complexed with Its Cognate tRNA

Title: Crystallization and Preliminary X-Ray Crystallographic Analysis of Yeast Tyrosyl-tRNA Synthetase Complexed with Its Cognate tRNA

Volume: 13 Issue: 4

Author(s): Yoshio Kusakabe, Satoshi Ohno, Nobutada Tanaka, Masashi Nakamura, Masaru Tsunoda, Tomohisa Moriguchi, Norio Asai, Mitsuo Sekine, Takashi Yokogawa, Kazuya Nishikawa and Kazuo T. Nakamura

Affiliation:

Keywords: aminoacyl-tRNA synthetase, crystallization, tRNA, TyrRS

Abstract: Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNATyr. The crystals belong to a tetragonal space group P41212 with cell dimensions of a = b = 63.85 Å, and c = 330.3 Å. The asymmetric unit contains one molecule each of yTyrRS and tRNATyr (one-half of a 2:2 complex). Xray diffraction data have been collected up to 2.5 Å resolution.

Cite this article as:

Kusakabe Yoshio, Ohno Satoshi, Tanaka Nobutada, Nakamura Masashi, Tsunoda Masaru, Moriguchi Tomohisa, Asai Norio, Sekine Mitsuo, Yokogawa Takashi, Nishikawa Kazuya and Nakamura T. Kazuo, Crystallization and Preliminary X-Ray Crystallographic Analysis of Yeast Tyrosyl-tRNA Synthetase Complexed with Its Cognate tRNA, Protein & Peptide Letters 2006; 13 (4) . https://dx.doi.org/10.2174/092986606775974465