The Early Events of α-Synuclein Oligomerization Revealed by Photo- Induced Cross-Linking

Title: The Early Events of α-Synuclein Oligomerization Revealed by Photo- Induced Cross-Linking

Volume: 13 Issue: 4

Author(s): Hong-Tao Li, Xiao-Jing Lin, Yuan-Yuan Xie and Hong-Yu Hu

Affiliation:

Keywords: α-synuclein, oligomerization, PICUP, dimmer, trimer

Abstract: Assembly of α-synuclein (α-Syn) into neurotoxic oligomers and fibrils is an important pathogenic feature of Parkinsons disease. Studying the early events of α-Syn aggregation, such as oligomerization and nucleation, is indispensable to understanding the complicated process. Here, photo-induced cross-linking of unmodified proteins (PICUP) technique is applied to elucidate the early-stage oligomerization of α-Syn. Results show that α-Syn in solution exhibits a mixture of various species, including at least monomers, dimers and trimers. Aggregation of α-Syn probably originates from the dimeric and trimeric seeds. Furthermore, the N-terminal amphipathic region is proposed to be required for the oligomerization (dimerization and trimerization) process. This observation may extend our knowledge on the early events of α-Syn aggregation and the neurotoxic aggregation species.

Cite this article as:

Li Hong-Tao, Lin Xiao-Jing, Xie Yuan-Yuan and Hu Hong-Yu, The Early Events of α-Synuclein Oligomerization Revealed by Photo- Induced Cross-Linking, Protein & Peptide Letters 2006; 13 (4) . https://dx.doi.org/10.2174/092986606775974384