Identification and Characterization of Trichoplusia ni Furin Truncated Mutant

Lie      Wang; Dan      Xu; Ming      Qian; Guanzhen      Yang; Xiangfu      Wu

doi:10.2174/092986606775101553

Abstract

We report A new prohormone convertase gene of insect origin, Trichoplusia ni furin, which was cloned from BTI-Tn-5B-4 (Tn5) insect cells. We constructed A truncated mutant that lacked Cys-rich repeated segments. Using baculovirus expression system and standard enzymatic assay, we obtained recombinant Tn furin and evaluated aspects of its function.

Keywords: Trichoplusia ni furin, baculovirus, Cys-rich repeated segment, Tn -5B1-4 insect cells

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Protein & Peptide Letters

Title: Identification and Characterization of Trichoplusia ni Furin Truncated Mutant

Volume: 13 Issue: 2

Author(s): Lie Wang, Dan Xu, Ming Qian, Guanzhen Yang and Xiangfu Wu

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Keywords: Trichoplusia ni furin, baculovirus, Cys-rich repeated segment, Tn -5B1-4 insect cells

Abstract: We report A new prohormone convertase gene of insect origin, Trichoplusia ni furin, which was cloned from BTI-Tn-5B-4 (Tn5) insect cells. We constructed A truncated mutant that lacked Cys-rich repeated segments. Using baculovirus expression system and standard enzymatic assay, we obtained recombinant Tn furin and evaluated aspects of its function.

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Wang Lie, Xu Dan, Qian Ming, Yang Guanzhen and Wu Xiangfu, Identification and Characterization of Trichoplusia ni Furin Truncated Mutant, Protein & Peptide Letters 2006; 13 (2) . https://dx.doi.org/10.2174/092986606775101553