A Nonradioactive Assay for Poly(A)-Specific Ribonuclease Activity by Methylene Blue Colorimetry

Title: A Nonradioactive Assay for Poly(A)-Specific Ribonuclease Activity by Methylene Blue Colorimetry

Volume: 13 Issue: 2

Author(s): Yuan Cheng, Wei-Feng Liu, Yong-Bin Yan and Hai-Meng Zhou

Affiliation:

Keywords: Enzyme assay, Poly(A)-specific ribonuclease, Methylene blue colorimetry, Enzyme inhibitor

Abstract: A simple nonradioactive assay, which was based on the specific shift of the absorbance maximum of methylene blue induced by its intercalation into poly(A) molecules, was developed for poly(A)-specific ribonuclease (PARN). A good linear relationship was found between the absorbance at 662 nm and the poly(A) concentration. The assay conditions, including the concentration of methylene blue, the incubation temperature and time, and the poly(A) concentration were evaluated and optimized.

Cite this article as:

Cheng Yuan, Liu Wei-Feng, Yan Yong-Bin and Zhou Hai-Meng, A Nonradioactive Assay for Poly(A)-Specific Ribonuclease Activity by Methylene Blue Colorimetry, Protein & Peptide Letters 2006; 13 (2) . https://dx.doi.org/10.2174/092986606775101580