Observations of the Mechanisms of Electromediated DNA Uptake - From Vesicles to Tissues

Title: Observations of the Mechanisms of Electromediated DNA Uptake - From Vesicles to Tissues

Volume: 10 Issue: 4

Author(s): Muriel Golzio, Jean-Michel Escoffre, Thomas Portet, Chloe Mauroy, Justin Teissie, David S. Dean and Marie-Pierre Rols

Affiliation:

Keywords: Delivery, electric field, electroporation, gene transfer, membrane, microscopy

Abstract: We discuss experimental observations of DNA uptake induced by electropermeabilisation. First we describe how experiments on giant unilamellar vesicles can be used to understand the effect of electric fields on lipid membranes and the associated transfer of DNA across the plasma membrane. We then discuss how DNA interacts with electropermeabilised cells in culture and how gene expression is correlated to observations of interactions between DNA and the cell membrane. Finally we discuss how DNA uptake occurs and can be optimised for the most medically relevant case of tissues.

Cite this article as:

Golzio Muriel, Escoffre Jean-Michel, Portet Thomas, Mauroy Chloe, Teissie Justin, S. Dean David and Rols Marie-Pierre, Observations of the Mechanisms of Electromediated DNA Uptake - From Vesicles to Tissues, Current Gene Therapy 2010; 10 (4) . https://dx.doi.org/10.2174/156652310791823461