Urea-Gradient Protein Refolding in Size Exclusion Chromatography

Chaozhan      Wang; Yan      Cheng

doi:10.2174/138920110791112004

Abstract

Protein refolding using urea gradient size exclusion chromatography (UGSEC) is a new version of conventional SEC refolding, which incorporates urea gradient into the SEC. Operating factors, mainly the urea gradient length and the final urea concentration in the gradient, are discussed in detail. Recent applications of UGSEC, including refolding of recombinant human granulocyte colony stimulating factor by UGSEC, is shown as an example.

Keywords: Urea gradient, size exclusion chromatography, protein refolding, recombinant human granulocyte colony stimulating factor

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Current Pharmaceutical Biotechnology

Title: Urea-Gradient Protein Refolding in Size Exclusion Chromatography

Volume: 11 Issue: 3

Author(s): Chaozhan Wang and Yan Cheng

Affiliation:

Keywords: Urea gradient, size exclusion chromatography, protein refolding, recombinant human granulocyte colony stimulating factor

Abstract: Protein refolding using urea gradient size exclusion chromatography (UGSEC) is a new version of conventional SEC refolding, which incorporates urea gradient into the SEC. Operating factors, mainly the urea gradient length and the final urea concentration in the gradient, are discussed in detail. Recent applications of UGSEC, including refolding of recombinant human granulocyte colony stimulating factor by UGSEC, is shown as an example.

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Wang Chaozhan and Cheng Yan, Urea-Gradient Protein Refolding in Size Exclusion Chromatography, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791112004