Recent Developments in Isothermal Titration Calorimetry Label Free Screening

Title: Recent Developments in Isothermal Titration Calorimetry Label Free Screening

Volume: 12 Issue: 8

Author(s): William B. Peters, Verna Frasca and Richard K. Brown

Affiliation:

Abstract: Isothermal titration calorimetry (ITC) is a label free technique used for direct detection of biological interactions by measurement of the heat given off or taken up during the reaction. In this article we will introduce the ITC technique and review two applications of ITC in drug discovery; small molecule/protein interactions and enzyme kinetics. We will also describe the characteristics of a new miniaturized, ultrasensitive calorimeter. This new microcalorimetry system reduces the quantity of protein (or other macromolecule sample) required to obtain a complete thermodynamic profile (n, K, ΔH and ΔS) by up to 7-fold. The reduction in required sample quantities allows ITC to be effectively utilized at earlier stages of the drug discovery and development process.

Cite this article as:

Peters B. William, Frasca Verna and Brown K. Richard, Recent Developments in Isothermal Titration Calorimetry Label Free Screening, Combinatorial Chemistry & High Throughput Screening 2009; 12 (8) . https://dx.doi.org/10.2174/138620709789104889