A Simple Technique to Establish a Long-Term Adenovirus Mediated Gene Transfer to the Heart of Newborn Mice

Title: A Simple Technique to Establish a Long-Term Adenovirus Mediated Gene Transfer to the Heart of Newborn Mice

Volume: 9 Issue: 2

Author(s): Marina Jerebtsova, Xuehai Ye and Patricio E. Ray

Affiliation:

Keywords: Recombinant adenoviral vector, long term gene transfer, newborn mouse, heart, cardiomyocytes

Abstract: Previous studies using different techniques have shown that adenoviral-mediated gene transfer to different tissues, including the kidney, is more efficient in neonatal mice. In this study, we report a simple technique that allows an efficient and long term expression of β-galactosidase (β-gal) in the heart of newborn mice. Newborn and adult C57BL6/J mice were subjected to a single retro-orbital venous plexus injection of recombinant adenoviral vectors (rAd) (2 x 10 particles/ g body weight) carrying the lac Z gene. Seven days after the injection, positive β-gal staining was systematically observed in the heart, lung, intestine, liver, kidney and spleen of newborn mice. However, only the heart showed persistent expression of β-gal one year after the initial injection. In contrast, adult mice showed only significant but transient β- gal expression mainly in the liver. In summary, we have found that a single retro-orbital intravenous injection can be used to establish a long-term adenoviral-mediated gene transfer to cardiac cells of newborn mice.

Cite this article as:

Jerebtsova Marina, Ye Xuehai and Ray E. Patricio, A Simple Technique to Establish a Long-Term Adenovirus Mediated Gene Transfer to the Heart of Newborn Mice, Cardiovascular & Hematological Disorders-Drug Targets 2009; 9 (2) . https://dx.doi.org/10.2174/187152909788488645