Biological Efficacy of Ionizing Radiation Sources on 3D Organotypic Tissue Slices Assessed by Fluorescence Microscopy

Title:Biological Efficacy of Ionizing Radiation Sources on 3D Organotypic Tissue Slices Assessed by Fluorescence Microscopy

Volume: 17

Author(s): Victoria Shestakova, Anna Smirnova, Anna Yakimova, Sergey Koryakin, Denis Baranovskii, Vyacheslav Saburov, Elena Yatsenko, Alexander Moiseev, Sergey Ivanov, Ekaterina Smirnova, Svetlana Belkina, Grigory Demyashkin, Lyudmila Komarova, Peter Shegay, Andrey Kaprin and Ilya Klabukov*

Affiliation:

• Department of Regenerative Medicine, National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Obninsk, Russia
• Obninsk Institute for Nuclear Power Engineering of the National Research Nuclear University MEPhI, Obninsk, Russia
• Patrice Lumumba Peoples’ Friendship University of Russia (RUDN University), Moscow, Russia

Abstract: Objective: Traditional cell-based radiobiological methods are inadequate for assessing the toxicity of ionizing radiation exposure in relation to the microstructure of the extracellular matrix. Organotypic tissue slices preserve the spatial organization observed in vivo, making the tissue easily accessible for visualization and staining. This study aims to explore the use of fluorescence microscopy of physiologically compatible 3D tissue cultures to assess the effects of ionizing radiation.

Methods: Organotypic tissue slices were obtained by vibratome, and their mechanical properties were studied. Slices were exposed by two ionizing radiation sources; electron beams (80 Gy and 4 Gy), and soft gamma irradiation (80 Gy and 4 Gy). Two tissue culture protocols were used: the standard (37°C), and hypothermic (30°C) conditions. A qualitative analysis of cell viability in organotypic tissue slices was performed using fluorescent dyes and standard laser confocal microscopy.

Results: Biological dosimetry is represented by differentially stained 200-µm thick organotypic tissue sections related to living and dead cells and cell metabolic activity.

Conclusion: Our results underscore the ability of fluorescence laser scanning confocal microscopy to rapidly assess the radiobiological effects of ionizing radiation in vitro on 3D organotypic tissue slices.

Cite this article as:

Shestakova Victoria, Smirnova Anna, Yakimova Anna, Koryakin Sergey, Baranovskii Denis, Saburov Vyacheslav, Yatsenko Elena, Moiseev Alexander, Ivanov Sergey, Smirnova Ekaterina, Belkina Svetlana, Demyashkin Grigory, Komarova Lyudmila, Shegay Peter, Kaprin Andrey and Klabukov Ilya*, Biological Efficacy of Ionizing Radiation Sources on 3D Organotypic Tissue Slices Assessed by Fluorescence Microscopy, Current Radiopharmaceuticals 2024; 17 () . https://dx.doi.org/10.2174/0118744710293570240419110322

DOI https://dx.doi.org/10.2174/0118744710293570240419110322	Print ISSN 1874-4710
Publisher Name Bentham Science Publisher	Online ISSN 1874-4729