Understanding the Molecular Mechanisms of H2's Regulatory Effect on miR-29s for Brain Protection during Deep Hypothermic Circulatory Arrest

Title:Understanding the Molecular Mechanisms of H2's Regulatory Effect on miR-29s for Brain Protection during Deep Hypothermic Circulatory Arrest

Volume: 27

Author(s): Hongmei Li, Li Shen*, Junwen Ge, Hongyun Li, Feng Chen, Xiaolong Chen and Haifa Hong

Affiliation:

• Department of Cardiothoracic Surgery, Shanghai Children's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200062, China

Abstract: Introduction: Research regarding post-operative brain protection after deep hypothermic circulatory arrest (DHCA) has gained attracted significant attention. We previously demonstrated that hydrogen can significantly reverse DHCA-induced brain damage.

Method: In the current research, we have established the DHCA model successfully using a modified four-vessel occlusion method and injected miR-29s compounds into the hippocampal tissue of rats.

Result: We were surprised to find hydrogen increased miR-29s expression in the hippocampal tissue of a DHCA rat model. The administration of agomiR-29s counteracted DHCA-induced hippocampal tissue injury, while the antamiR-29s had the opposite effects.

Conclusion: Based on the above facts, the brain protection mechanism of hydrogen in DHCAtreated rats may be related to the upregulation of miR-29s, which can exert its beneficial effects by alleviating apoptosis, inflammation, and oxidation.

Cite this article as:

Li Hongmei, Shen Li*, Ge Junwen, Li Hongyun, Chen Feng, Chen Xiaolong and Hong Haifa, Understanding the Molecular Mechanisms of H2's Regulatory Effect on miR-29s for Brain Protection during Deep Hypothermic Circulatory Arrest, Combinatorial Chemistry & High Throughput Screening 2024; 27 () . https://dx.doi.org/10.2174/0113862073281209231227044205