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Research Article

P53-induced GAP-43 Upregulation in Primary Cortical Neurons of Rats

Author(s): Tianxia Li, Yuexin Jia, Junxian Fu, Zhuo Fu, Zhidong Qiao, Xiaoyang Liu, Ting Lv, Rong Tang and Guanglu Yang*

Volume 31, Issue 3, 2024

Published on: 26 January, 2024

Page: [229 - 235] Pages: 7

DOI: 10.2174/0109298665263864231221071712

Abstract

Objectives: In this study, we employed an in vitro culturing technique to investigate the impact of p53 on the modulation of growth-associated protein-43 (GAP-43) within the primary cortical neurons of rat specimens.

Methods: (1) Within the first 24 hours after birth, the bilateral cortex was extracted from newborn Wistar rats and primary cortical neurons were cultured and identified. (2) The changes in the mRNA and protein expressions of GAP-43 induced by p53 in rat primary cortical neurons cultured in vitro were identified utilizing real-time polymerase chain reaction and western blot techniques.

Results: (1) Lentiviral transfection of p53 within primary cortical neurons of rats elicited elevated levels of both mRNA and protein expressions of GAP-43, consequently culminating in a noteworthy augmentation of p53 expression. (2) The introduction of a p53 inhibitor in rat primary cortical neurons resulted in a reduction in both mRNA and protein expressions of GAP-43.

Conclusion: Within primary rat cortical neurons, p53 has the potential to prompt an augmentation in both the transcriptional and protein expression levels of the GAP-43 protein.

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Graphical Abstract

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