Login Register Cart 0
Generic placeholder image

Current Drug Therapy

Editor-in-Chief

ISSN (Print): 1574-8855
ISSN (Online): 2212-3903

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Pharmacological Investigation of Anticonvulsant Effect of Artemisia Vulgaris on Pentylenetetrazole-Induced Epileptic Seizures in Zebrafish Experimental Model

In Press, (this is not the final "Version of Record"). Available online 08 January, 2024
Author(s): Mahima Sharma, Godlaveti Vijay Narasimha Kumar and Pankaj Gupta*
Published on: 08 January, 2024

DOI: 10.2174/0115748855268042231130041958

Price: $95

Abstract

Background: Epilepsy is the most common neurological disorder, characterized by abnormal, unpredictable, and recurrent seizures. In the alternative medicine system, Artemisia vulgaris has been used to treat epileptic symptoms since its inception. However, scientific evidence concerning its effect on the treatment of epileptic seizures is not available.

Objective: The objective of the study is to evaluate the anti-convulsant potential of Artemisia vulgaris in zebrafish experimental models.

Methods: Zebrafish larvae and adult zebrafish were used as experimental models. Briefly, larvae and adult zebrafish were exposed to 0.5% of the test medicine Artemisia vulgaris mother tincture (ϕ) and its potencies (6CH, 30CH), and valproic acid for 1 hour. After 1 hour of exposure, they were exposed to pentylenetetrazole to record different seizure scores from 1 to 5 using ANY maze video tracking software.

Results: Artemisia vulgaris 30CH in zebrafish larvae and Artemisia vulgaris 6CH and 30CH in adult zebrafish delayed the latency score from score 1 to score 5 and were found effective against PTZinduced locomotor activity and seizure duration and intensity.

Conclusion: In conclusion, Artemisia vulgaris (6CH and 30CH) has the potential to be a novel treatment for symptomatic epileptic seizures and could be a potential alternative drug candidate for symptomatic treatment of epilepsy.

[1]
Fisher RS, Harding G, Erba G, Barkley GL, Wilkins A. Photic- and pattern-induced seizures: A review for the epilepsy foundation of America working group. Epilepsia 2005; 46(9): 1426-41.
[http://dx.doi.org/10.1111/j.1528-1167.2005.31405.x] [PMID: 16146439]
[2]
Mussulini BHM, Leite CE, Zenki KC, Moro L, Baggio S, Rico EP, et al. Seizures induced by pentylenetetrazole in the adult zebrafish: A detailed behavioral characterization. PLoS ONE 2013.
[http://dx.doi.org/10.1371/journal.pone.0054515]
[3]
Afrikanova T, Serruys ASK, Buenafe OEM, et al. Validation of the zebrafish pentylenetetrazol seizure model: Locomotor versus electrographic responses to antiepileptic drugs. PLoS One 2013; 8(1): e54166.
[http://dx.doi.org/10.1371/journal.pone.0054166] [PMID: 23342097]
[4]
Surges R, Thijs RD, Tan HL, Sander JW. Sudden unexpected death in epilepsy: Risk factors and potential pathomechanisms. Nat Rev Neurol 2009; 5(9): 492-504.
[http://dx.doi.org/10.1038/nrneurol.2009.118] [PMID: 19668244]
[5]
Begley CE, Famulari M, Annegers JF, et al. The cost of epilepsy in the United States: An estimate from population-based clinical and survey data. Epilepsia 2000; 41(3): 342-51.
[http://dx.doi.org/10.1111/j.1528-1157.2000.tb00166.x] [PMID: 10714408]
[6]
Heron SE, Scheffer IE, Berkovic SF, Dibbens LM, Mulley JC. Channelopathies in idiopathic epilepsy. Neurotherapeutics 2007; 4(2): 295-304.
[http://dx.doi.org/10.1016/j.nurt.2007.01.009] [PMID: 17395140]
[7]
Holmes GL. Cognitive impairment in epilepsy: The role of network abnormalities. Epileptic Disord 2015; 17(2): 101-16.
[http://dx.doi.org/10.1684/epd.2015.0739] [PMID: 25905906]
[8]
Sucher NJ, Carles MC. A pharmacological basis of herbal medicines for epilepsy. Epilepsy Behav 2015; 52(Pt B): 308-18.
[http://dx.doi.org/10.1016/j.yebeh.2015.05.012] [PMID: 26074183]
[9]
Golechha M, Bhatia J, Arya DS. Hydroalcoholic extract of Emblica officinalis Gaertn. affords protection against PTZ-induced seizures, oxidative stress and cognitive impairment in rats. Indian J Exp Biol 2010; 48(5): 474-8.
[PMID: 20795364]
[10]
Raza M, Iqbal Choudhary M. Medicinal plants with anticonvulsant activities.Studies in Natural Products Chemistry. Elsevier 2000; pp. 507-53.
[11]
Preux PM, Tiemagni F, Fodzo L, et al. Antiepileptic therapies in the mifi province in cameroon. Epilepsia 2000; 41(4): 432-9.
[http://dx.doi.org/10.1111/j.1528-1157.2000.tb00185.x] [PMID: 10756409]
[12]
Duke JA. Database of biologically active phytochemicals and their activity. Boca Raton, Fla: CRC Press 1992.
[13]
Hershoff A, Rotelli A. Herbal Remedies: A quick and easy guide to common disorders and their herbal remedies. Avery 2001.
[14]
Abiri R, Silva ALM, de Mesquita LSS, de Mesquita JWC, Atabaki N, de Almeida EB, et al. Towards a better understanding of Artemisia vulgaris: Botany, phytochemistry, pharmacological and biotechnological potential. Food Res Int 2018; 109: 403-15.
[15]
Saraji M, Khayamian T, Hashemian Z, Aslipashaki SN, Talebi M. Determination of artemisinin in Artemisia species by hollow fiber-based liquid-phase microextraction and electrospray ionization-ion mobility spectrometry. Anal Methods 2013; 5(16): 4190-5.
[http://dx.doi.org/10.1039/c3ay40701b]
[16]
Corrêa-Ferreira ML, Verdan MH, dos Reis Lívero FA, et al. Inulin-type fructan and infusion of Artemisia vulgaris protect the liver against carbon tetrachloride-induced liver injury. Phytomedicine 2017; 24: 68-76.
[http://dx.doi.org/10.1016/j.phymed.2016.11.017] [PMID: 28160864]
[17]
Nguyen HTT, Nguyen HT, Islam MZ, et al. Pharmacological characteristics of Artemisia vulgaris L. in isolated porcine basilar artery. J Ethnopharmacol 2016; 182: 16-26.
[http://dx.doi.org/10.1016/j.jep.2016.02.009] [PMID: 26875644]
[18]
Soon L, Ng PQ, Chellian J, et al. Therapeutic potential of Artemisia vulgaris: An insight into underlying immunological mechanisms. J Environ Pathol Toxicol Oncol 2019; 38(3): 205-16.
[http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.2019029397] [PMID: 31679308]
[19]
Ali S, Ejaz M, Dar KK, et al. Evaluation of chemopreventive and chemotherapeutic effect of Artemisia vulgaris extract against diethylnitrosamine induced hepatocellular carcinogenesis in Balb C mice. Braz J Biol 2020; 80(3): 484-96.
[http://dx.doi.org/10.1590/1519-6984.185979] [PMID: 31691741]
[20]
De Sousa DP, Nobrega FFF, De Morais LCSL, De Almeida RN. Evaluation of the anticonvulsant activity of terpinen-4-ol. Zeitschrift fur Naturforsch-Sect C. J Biosci 2009; 64(1-2): 1-5.
[21]
Elisabetsky E, Marschner J, Onofre Souza D. Effects of linalool on glutamatergic system in the rat cerebral cortex. Neurochem Res 1995; 20(4): 461-5.
[http://dx.doi.org/10.1007/BF00973103] [PMID: 7651584]
[22]
Clarke JH. A dictionary of practical materia medica Artemisia Vulgaris. London: Homoeopathic Pub Co 1908; 2: pp. 1852-931.
[23]
Sourbron J, Schneider H, Kecskés A, et al. Serotonergic modulation as effective treatment for dravet syndrome in a zebrafish mutant model. ACS Chem Neurosci 2016; 7(5): 588-98.
[http://dx.doi.org/10.1021/acschemneuro.5b00342] [PMID: 26822114]
[24]
Parng C, Seng WL, Semino C, McGrath P. Zebrafish: A preclinical model for drug screening. Assay Drug Dev Technol 2002; 1(1): 41-8.
[http://dx.doi.org/10.1089/154065802761001293] [PMID: 15090155]
[25]
Berghmans S, Hunt J, Roach A, Goldsmith P. Zebrafish offer the potential for a primary screen to identify a wide variety of potential anticonvulsants. Epilepsy Res 2007; 75(1): 18-28.
[http://dx.doi.org/10.1016/j.eplepsyres.2007.03.015] [PMID: 17485198]
[26]
Westerfield M. The zebrafish book: A guide for the laboratory use of zebrafish (Danio rerio). (5th ed.), Eugene: University of Oregon Press 2007.
[27]
Choo BKM, Kundap UP, Kumari Y, Hue SM, Othman I, Shaikh MF. Orthosiphon stamineus leaf extract affects TNF-α and seizures in a zebrafish model. Front Pharmacol 2018; 9: 139.
[http://dx.doi.org/10.3389/fphar.2018.00139] [PMID: 29527169]
[28]
Baraban SC, Taylor MR, Castro PA, Baier H. Pentylenetetrazole induced changes in zebrafish behavior, neural activity and c-fos expression. Neuroscience 2005; 131(3): 759-68.
[http://dx.doi.org/10.1016/j.neuroscience.2004.11.031] [PMID: 15730879]
[29]
Alfaro JM, Ripoll-Gómez J, Burgos JS. Kainate administered to adult zebrafish causes seizures similar to those in rodent models. Eur J Neurosci 2011; 33(7): 1252-5.
[http://dx.doi.org/10.1111/j.1460-9568.2011.07622.x] [PMID: 21375600]
[30]
Blaser RE, Rosemberg DB. Measures of anxiety in zebrafish (Danio rerio): Dissociation of black/white preference and novel tank test. PLoS One 2012; 7(5): e36931.
[http://dx.doi.org/10.1371/journal.pone.0036931] [PMID: 22615849]
[31]
Judzentiene A, Garjonyte R. Compositional variability and toxic activity of Mugwort ( Artemisia vulgaris ) essential oils. Nat Prod Commun 2016; 11(9): 1934578X1601100.
[http://dx.doi.org/10.1177/1934578X1601100942] [PMID: 30807041]
[32]
Sailike B, Omarova Z, Jenis J, Adilbayev A, Akbay B, Askarova S, et al. Neuroprotective and anti-epileptic potentials of genus Artemisia L. Front Pharmacol 2022; 19(13): 1021501.
[33]
Pineda R, Beattie CE, Hall CW. Recording the adult zebrafish cerebral field potential during pentylenetetrazole seizures. J Neurosci Methods 2011; 200(1): 20-8.
[http://dx.doi.org/10.1016/j.jneumeth.2011.06.001] [PMID: 21689682]
[34]
Duy PQ, Berberoglu MA, Beattie CE, Hall CW. Cellular responses to recurrent pentylenetetrazole-induced seizures in the adult zebrafish brain. Neuroscience 2017; 349: 118-27.
[http://dx.doi.org/10.1016/j.neuroscience.2017.02.032] [PMID: 28238851]
[35]
Hortopan GA, Dinday MT, Baraban SC. Spontaneous seizures and altered gene expression in GABA signaling pathways in a mind bomb mutant zebrafish. J Neurosci 2010; 30(41): 13718-28.
[http://dx.doi.org/10.1523/JNEUROSCI.1887-10.2010] [PMID: 20943912]
[36]
Vermoesen K, Serruys ASK, Loyens E, et al. Assessment of the convulsant liability of antidepressants using zebrafish and mouse seizure models. Epilepsy Behav 2011; 22(3): 450-60.
[http://dx.doi.org/10.1016/j.yebeh.2011.08.016] [PMID: 21962757]
[37]
Orellana-Paucar AM, Serruys ASK, Afrikanova T, et al. Anticonvulsant activity of bisabolene sesquiterpenoids of Curcuma longa in zebrafish and mouse seizure models. Epilepsy Behav 2012; 24(1): 14-22.
[http://dx.doi.org/10.1016/j.yebeh.2012.02.020] [PMID: 22483646]
[38]
Siebel AM, Piato AL, Schaefer IC, Nery LR, Bogo MR, Bonan CD. Antiepileptic drugs prevent changes in adenosine deamination during acute seizure episodes in adult zebrafish. Pharmacol Biochem Behav 2013; 104(1): 20-6.
[http://dx.doi.org/10.1016/j.pbb.2012.12.021] [PMID: 23287800]
[39]
Siebel AM, Menezes FP, da Costa Schaefer I, Petersen BD, Bonan CD. Rapamycin suppresses PTZ-induced seizures at different developmental stages of zebrafish. Pharmacol Biochem Behav 2015; 139(Pt B): 163-8.
[http://dx.doi.org/10.1016/j.pbb.2015.05.022] [PMID: 26051026]
[40]
Winter MJ, Redfern WS, Hayfield AJ, Owen SF, Valentin JP, Hutchinson TH. Validation of a larval zebrafish locomotor assay for assessing the seizure liability of early-stage development drugs. J Pharmacol Toxicol Methods 2008; 57(3): 176-87.
[http://dx.doi.org/10.1016/j.vascn.2008.01.004] [PMID: 18337127]
[41]
Chang P, Orabi B, Deranieh RM, et al. The antiepileptic drug valproic acid and other medium-chain fatty acids acutely reduce phosphoinositide levels independently of inositol in Dictyostelium. Dis Model Mech 2012; 5(1): 115-24.
[http://dx.doi.org/10.1242/dmm.008029] [PMID: 21876211]
[42]
Banote RK, Koutarapu S, Chennubhotla KS, Chatti K, Kulkarni P. Oral gabapentin suppresses pentylenetetrazole-induced seizure-like behavior and cephalic field potential in adult zebrafish. Epilepsy Behav 2013; 27(1): 212-9.
[http://dx.doi.org/10.1016/j.yebeh.2013.01.018] [PMID: 23466252]
[43]
Cachat J, Stewart A, Utterback E, et al. Three-dimensional neurophenotyping of adult zebrafish behavior. PLoS One 2011; 6(3): e17597.
[http://dx.doi.org/10.1371/journal.pone.0017597] [PMID: 21408171]
[44]
Rosa LV, Ardais AP, Costa FV, et al. Different effects of caffeine on behavioral neurophenotypes of two zebrafish populations. Pharmacol Biochem Behav 2018; 165: 1-8.
[http://dx.doi.org/10.1016/j.pbb.2017.12.002] [PMID: 29241648]
[45]
Canzian J, Franscescon F, Müller TE, Stefanello FV, Souza TP, Rosa LV, et al. Stress increases susceptibility to pentylenetetrazole-induced seizures in adult zebrafish. Epilepsy Behav 2021; 114(Pt A): 107557.
[http://dx.doi.org/10.1016/j.yebeh.2020.107557]
[46]
Fontana BD, Ziani PR, Canzian J, et al. Taurine protects from pentylenetetrazole-induced behavioral and neurochemical changes in zebrafish. Mol Neurobiol 2019; 56(1): 583-94.
[http://dx.doi.org/10.1007/s12035-018-1107-8] [PMID: 29748917]

Rights & Permissions Print Cite
© 2025 Bentham Science Publishers | Privacy Policy