New Methods of Affinity Electrophoresis

Title: New Methods of Affinity Electrophoresis

Volume: 2 Issue: 3

Author(s): Bao-Shiang Lee, Sangeeth Krishnanchettiar, Syed S. Lateef and Shalini Gupta

Affiliation:

Keywords: Proteomics, Affinity electrophoresis, Glycoproteins, Mass spectrometry, Avidin, Biotin, MALDI-TOF MS, ICAT

Abstract: Several alternative methods of conducting affinity electrophoresis are described, including countercurrent affinity electrophoresis, antibody affinity electrophoresis, functional affinity electrophoresis, and avidin functional affinity electrophoresis. The basis of these new techniques is to catch the prey molecules while they migrate electrophoretically through the immobilized bait molecules region of the polyacrylamide gel. Potential applications of these techniques will be indicated.

Cite this article as:

Lee Bao-Shiang, Krishnanchettiar Sangeeth, Lateef S. Syed and Gupta Shalini, New Methods of Affinity Electrophoresis, Current Analytical Chemistry 2006; 2 (3) . https://dx.doi.org/10.2174/157341106777698206