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Current Drug Safety

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ISSN (Print): 1574-8863
ISSN (Online): 2212-3911

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Research Article

Evaluation of the Antioxidant Effects of Berberine against Sodium Nitriteinduced Oxidative Injury in the Rat Liver

Author(s): Morteza Akhzari, Sajad Jalili, Sajad Rashno, Masoumeh Akmali, Hossein Foruozandeh and Mahdi Barazesh*

Volume 19, Issue 1, 2024

Published on: 24 February, 2023

Page: [51 - 60] Pages: 10

DOI: 10.2174/1574886318666230119093541

Price: $65

Abstract

Objectives: Berberine is a plant derived alkaloid present in many plants that may has ameliorating potential influences against inflammatory and oxidative conditions. The current study aimed to evaluate the possible protective activity of berberine and investigate its probable mechanisms against sodium nitrite toxicity in the liver.

Methods: Forty male rats were divided into five groups. Group one, as the control group, received normal saline, group two received berberine (100 mg.kg-1), and group three received sodium nitrite (80 mg.kg-1). Groups four and five received berberine in doses of 50 and 100 mg.kg-1, respectively, and sodium nitrite (80 mg.kg-1) was given orally. All the doses were orally administrated for two months. Then, at the end of the 60th day, the animals were sacrificed, and the liver homogenate was prepared. For evaluating the oxidative injury the levels of albumin (ALB) and aspartate transaminase (AST) in the serum and oxidative stress parameters in the liver were analyzed.

Results: Treatment of rats with sodium nitrite considerably increased the levels of serum AST and liver superoxide anion and significantly reduced the levels of serum ALB, hepatic superoxide dismutase (SOD), total antioxidant capacity (TAC), and catalase (CAT) activity in the liver tissue. Berberine treatment could ameliorate all these parameters dose dependently. Berberine at a dose of 100 mg.kg-1 had the best impact and reached the values of oxidative stress parameters to the normal level.

Conclusion: Our results demonstrated that berberine in a dose-dependent manner offered protection against sodium nitrite-induced oxidative injury in liver, which possibly reflects the antioxidant abilities of this alkaloid.

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[1]
Kalantari H, Foruozandeh H, Khodayar MJ, Siahpoosh A, Saki N, Kheradmand P. Antioxidant and hepatoprotective effects of Capparis spinosa L. fractions and Quercetin on tert-butyl hydroperoxide- induced acute liver damage in mice. J Tradit Complement Med 2018; 8(1): 120-7.
[http://dx.doi.org/10.1016/j.jtcme.2017.04.010] [PMID: 29321999]
[2]
Farahani A, Forouzandeh H, Kalantari H, et al. Role of oxidative stress in liver cancer. Clin Cancer Investig J 2017; 6(1): 1.
[http://dx.doi.org/10.4103/ccij.ccij_176_16]
[3]
Council NR. Nitrate and nitrite in drinking water. National Academies Press 1995.
[4]
Milkowski A, Garg HK, Coughlin JR, Bryan NS. Nutritional epidemiology in the context of nitric oxide biology: A risk–benefit evaluation for dietary nitrite and nitrate. Nitric Oxide 2010; 22(2): 110-9.
[http://dx.doi.org/10.1016/j.niox.2009.08.004] [PMID: 19748594]
[5]
Katabami K, Hayakawa M, Gando S. Severe methemoglobinemia due to sodium nitrite poisoning. Case Reports Emerg Med 2016; 2016: 1-3.
[http://dx.doi.org/10.1155/2016/9013816]
[6]
Xu DP, Li Y, Meng X, et al. Natural antioxidants in foods and medicinal plants: Extraction, assessment and resources. Int J Mol Sci 2017; 18(1): 96.
[http://dx.doi.org/10.3390/ijms18010096] [PMID: 28067795]
[7]
Preeti S, Prabhat U, Shardendu M, Ananya S, Suresh P. Berberine a potent substance for researcher: A Review. World J Pharm Pharm Sci 2015; 4(07): 547-73.
[8]
Ahmed T, Gilani AH, Abdollahi M, Daglia M, Nabavi SF, Nabavi SM. Berberine and neurodegeneration: A review of literature. Pharmacol Rep 2015; 67(5): 970-9.
[http://dx.doi.org/10.1016/j.pharep.2015.03.002] [PMID: 26398393]
[9]
Council NR. Committee for the Update of the Guide for the Care and Use of Laboratory Animals 2011. Guide for the Care and Use of Laboratory Animals 2011; pp. 8.
[10]
Babson AL, Shapiro PO, Williams PAR, Phillips GE. The use of a diazonium salt for the determination of glutamic-oxalacetic transaminase in serum. Clin Chim Acta 1962; 7(2): 199-205.
[http://dx.doi.org/10.1016/0009-8981(62)90010-4] [PMID: 13863676]
[11]
Louderback A. A new dye binding technic using bromcresol purple for determination of albumin in serum. Clin Chem 1968; 14: 793-4.
[12]
Zeb A, Ullah F. A simple spectrophotometric method for the determination of thiobarbituric acid reactive substances in fried fast foods. J Anal Methods Chem 2016; 2016: 9412767.
[http://dx.doi.org/10.1155/2016/9412767]
[13]
Mukherjee D, Manna M, Selvaraj S, Bhattacharya S, Homechoudhury S, Chakraborty A. Radiation induced effects on viability and antioxidant enzymes of crustaceans from different habitats. J Environ Biol 2010; 31(3): 251-4.
[14]
Surapaneni K, Venkataramana G. Status of lipid peroxidation, glutathione, ascorbic acid, vitamin E and antioxidant enzymes in patients with osteoarthritis. Indian J Med Sci 2007; 61(1): 9-14.
[http://dx.doi.org/10.4103/0019-5359.29592] [PMID: 17197733]
[15]
Rubio CP, Hernández-Ruiz J, Martinez-Subiela S, Tvarijonaviciute A, Ceron JJ. Spectrophotometric assays for Total antioxidant capacity (TAC) in dog serum: An update. BMC Vet Res 2016; 12(1): 166.
[http://dx.doi.org/10.1186/s12917-016-0792-7] [PMID: 27526688]
[16]
Sim Choi H, Woo Kim J, Cha YN, Kim C. A quantitative nitroblue tetrazolium assay for determining intracellular superoxide anion production in phagocytic cells. J Immunoassay Immunochem 2006; 27(1): 31-44.
[http://dx.doi.org/10.1080/15321810500403722] [PMID: 16450867]
[17]
Kunwar A, Priyadarsini K. Free radicals, oxidative stress and importance of antioxidants in human health. J Med Appl Sci 2011; 1(2): 53.
[18]
Christiansen LN, Tompkin RB, Shaparis AB, et al. Effect of sodium nitrite on toxin production by Clostridium botulinum in bacon. Appl Microbiol 1974; 27(4): 733-7.
[http://dx.doi.org/10.1128/am.27.4.733-737.1974] [PMID: 4596753]
[19]
Sen NP, Smith DC, Schwinghamer L. Formation of N-nitrosamines from secondary amines andnitrite in human and animal gastric juice. Food Cosmet Toxicol 1969; 7(4): 301-7.
[http://dx.doi.org/10.1016/S0015-6264(69)80366-4] [PMID: 5823975]
[20]
Forouzandeh H, Azemi ME, Rashidi I, Goudarzi M, Kalantari H. Study of the protective effect of Teucrium polium L. extract on acetaminophen-induced hepatotoxicity in mice. Iran J Pharm Res 2013; 12(1): 123-9.
[PMID: 24250580]
[21]
Beckman JS, Koppenol WH. Nitric oxide, superoxide, and peroxynitrite: The good, the bad, and ugly. Am J Physiol Cell Physiol 1996; 271(5): C1424-37.
[http://dx.doi.org/10.1152/ajpcell.1996.271.5.C1424] [PMID: 8944624]
[22]
Pacher P, Beckman JS, Liaudet L. Nitric oxide and peroxynitrite in health and disease. Physiol Rev 2007; 87(1): 315-424.
[http://dx.doi.org/10.1152/physrev.00029.2006] [PMID: 17237348]
[23]
Kono Y, Fridovich I. Superoxide radical inhibits catalase. J Biol Chem 1982; 257(10): 5751-4.
[http://dx.doi.org/10.1016/S0021-9258(19)83842-5] [PMID: 6279612]
[24]
Shou JW, Li XX, Tang YS, et al. Novel mechanistic insight on the neuroprotective effect of berberine: The role of PPARδ for antioxidant action. Free Radic Biol Med 2022; 181: 62-71.
[http://dx.doi.org/10.1016/j.freeradbiomed.2022.01.022] [PMID: 35093536]
[25]
Luo A, Fan Y. Antioxidant activities of berberine hydrochloride. J Med Plants Res 2011; 5(16): 3702-7.
[26]
An N, Zhang G, Li Y, et al. Promising antioxidative effect of berberine in cardiovascular diseases. Front Pharmacol 2022; 13: 865353.
[http://dx.doi.org/10.3389/fphar.2022.865353] [PMID: 35321323]
[27]
Akhzari M, Barazesh M, Jalili S. Berberine recovered oxidative stress induced by sodium nitrite in rat erythrocytes. Drug Metab Bioanal Lett 2022; 15(3): 192-201.
[28]
Ansari FA, Mahmood R. Sodium nitrite enhances generation of reactive oxygen species that decrease antioxidant power and inhibit plasma membrane redox system of human erythrocytes. Cell Biol Int 2016; 40(8): 887-94.
[http://dx.doi.org/10.1002/cbin.10628] [PMID: 27214747]
[29]
Hasanein P, Ghafari-Vahed M, Khodadadi I. Effects of isoquinoline alkaloid berberine on lipid peroxidation, antioxidant defense system, and liver damage induced by lead acetate in rats. Redox Rep 2017; 22(1): 42-50.
[http://dx.doi.org/10.1080/13510002.2016.1140406] [PMID: 26871196]
[30]
Eissa LA, Kenawy HI, El-Karef A, Elsherbiny NM, El-Mihi KA. Antioxidant and anti-inflammatory activities of berberine attenuate hepatic fibrosis induced by thioacetamide injection in rats. Chem Biol Interact 2018; 294: 91-100.
[http://dx.doi.org/10.1016/j.cbi.2018.08.016] [PMID: 30138605]
[31]
Janbaz KH, Gilani AH. Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents. Fitoterapia 2000; 71(1): 25-33.
[http://dx.doi.org/10.1016/S0367-326X(99)00098-2] [PMID: 11449466]
[32]
Li D, Wu M. Protective effect of berberine on CCl4 induced acute liver injury in mice. China Pharmacy 2009; 20(21): 1618-9.
[33]
Wang L, Sagada G, Wang C, et al. Berberine in fish nutrition: Impact on hepatoenteric health, antioxidative and immune status. Front Mar Sci 2022; 9: 967748.
[http://dx.doi.org/10.3389/fmars.2022.967748]
[34]
Rad SZK, Rameshrad M, Hosseinzadeh H. Toxicology effects of Berberis vulgaris (barberry) and its active constituent, berberine: A review. Iran J Basic Med Sci 2017; 20(5): 516-29.
[PMID: 28656087]
[35]
Kheir MM, Wang Y, Hua L, et al. Acute toxicity of berberine and its correlation with the blood concentration in mice. Food Chem Toxicol 2010; 48(4): 1105-10.
[http://dx.doi.org/10.1016/j.fct.2010.01.033] [PMID: 20138204]
[36]
Küpeli E, Koşar M, Yeşilada E, Başer KHC, Başer C. A comparative study on the anti-inflammatory, antinociceptive and antipyretic effects of isoquinoline alkaloids from the roots of Turkish Berberis species. Life Sci 2002; 72(6): 645-57.
[http://dx.doi.org/10.1016/S0024-3205(02)02200-2] [PMID: 12467905]
[37]
Yeşilada E, Küpeli E. Berberis crataegina DC. root exhibits potent anti-inflammatory, analgesic and febrifuge effects in mice and rats. J Ethnopharmacol 2002; 79(2): 237-48.
[http://dx.doi.org/10.1016/S0378-8741(01)00387-7] [PMID: 11801387]
[38]
Zhou JY, Zhou SW, Zhang KB, et al. Chronic effects of berberine on blood, liver glucolipid metabolism and liver PPARs expression in diabetic hyperlipidemic rats. Biol Pharm Bull 2008; 31(6): 1169-76.
[http://dx.doi.org/10.1248/bpb.31.1169] [PMID: 18520050]

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