Login Register Cart 0
Generic placeholder image

Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

Circ_0002762 Regulates Oncoprotein YBX1 in Cervical Cancer via mir-375 to Regulate the Malignancy of Cancer Cells

Author(s): Wei Zheng, Haixia Mu, Jing Chen, Chunjie Wang* and Li Hou*

Volume 30, Issue 2, 2023

Published on: 03 February, 2023

Page: [162 - 172] Pages: 11

DOI: 10.2174/0929866530666230104155209

Price: $65

Abstract

Background: Cervical carcinoma (CC) is the third most common cancer among females and the fourth leading cause of cancer-related death, which poses a serious threat to women's health. This study investigated the biological function and mechanism of circRNA circ_0002762 in the malignant progression of CC.

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was used to quantify circ_0002762, microRNA-375 (miR-375) and Y-box binding protein 1 (YBX1) mRNA expressions in CC tissues and cell lines. After circ_0002762 was overexpressed in CC cell lines, cell counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU) and wound healing assays were executed to probe cell growth and migration. Additionally, the targeting relationships between miR-375 and circ_0002762 or YBX1 3’-UTR were confirmed by dual-luciferase reporter assay and RNA immunoprecipitation assay. Western blot was adopted to examine YBX1 protein levels in CC cells.

Results: Circ_0002762 expression was raised in CC tissues and cell lines, and highly expressed circ_0002762 was associated with larger tumor size and lymph node metastasis of CC patients. Circ_0007262 overexpression markedly accelerated the proliferation and migration of CC cells. Besides, miR-375 was revealed to be a downstream target of circ_0002762, and miR-375 overexpression counteracted the promoting effects of circ_0002762 overexpression on CC cell viability and migration. YBX1 was identified as a target of miR-375, and circ_0002762 positively modulated YBX1 expressions through adsorbing miR-375.

Conclusion: Circ_0002762 promotes the progression of CC via sponging miR-375 and up-regulating YXB1 expression.

« Previous Next »
Graphical Abstract

[1]
Padilla-Mendoza, J.R.; Gómez-López, L.A.; López-Casamichana, M.; Azuara-Liceaga, E.I.; Cortés-Malagón, E.M.; López-Cánovas, L.; Reyes-Hernández, O.D.; Rodríguez, M.A.; Bonilla-Delgado, J.; López-Reyes, I. Human papillomavirus coinfection in the cervical intraepithelial lesions and cancer of mexican patients. BioMed Res. Int., 2020, 2020, 4542320.
[http://dx.doi.org/10.1155/2020/4542320] [PMID: 33274212]
[2]
Yadav, M.; Sethi, S.; Ramalingam, V.; Thulkar, S. Nodal metastasis of cervix carcinoma-as a probable cause of bone erosion. J. Cancer Res. Ther., 2019, 15(6), 1260-1264.
[http://dx.doi.org/10.4103/jcrt.JCRT_374_18] [PMID: 31898657]
[3]
Fleming, N.D.; Frumovitz, M.; Schmeler, K.M.; dos Reis, R.; Munsell, M.F.; Eifel, P.J.; Soliman, P.T.; Nick, A.M.; Westin, S.N.; Ramirez, P.T. Significance of lymph node ratio in defining risk category in node-positive early stage cervical cancer. Gynecol. Oncol., 2015, 136(1), 48-53.
[http://dx.doi.org/10.1016/j.ygyno.2014.11.010] [PMID: 25451695]
[4]
Shen, T.; Han, M.; Wei, G.; Ni, T. An intriguing RNA species—perspectives of circularized RNA. Protein Cell, 2015, 6(12), 871-880.
[http://dx.doi.org/10.1007/s13238-015-0202-0] [PMID: 26349458]
[5]
Zang, J.; Lu, D.; Xu, A. The interaction of circRNAs and RNA binding proteins: An important part of circRNA maintenance and function. J. Neurosci. Res., 2020, 98(1), 87-97.
[http://dx.doi.org/10.1002/jnr.24356] [PMID: 30575990]
[6]
Su, Y.; Lv, X.; Yin, W.; Zhou, L.; Hu, Y.; Zhou, A.; Qi, F. CircRNA Cdr1as functions as a competitive endogenous RNA to promote hepatocellular carcinoma progression. Aging, 2019, 11(19), 8183-8203.
[http://dx.doi.org/10.18632/aging.102312] [PMID: 31581132]
[7]
Chaichian, S.; Shafabakhsh, R.; Mirhashemi, S.M.; Moazzami, B.; Asemi, Z. Circular RNAs: A novel biomarker for cervical cancer. J. Cell. Physiol., 2020, 235(2), 718-724.
[http://dx.doi.org/10.1002/jcp.29009] [PMID: 31240697]
[8]
Cao, Y.; Li, J.; Jia, Y.; Zhang, R.; Shi, H. CircRNA circ_POLA2 promotes cervical squamous cell carcinoma progression via regulating miR-326/GNB1. Front. Oncol., 2020, 10, 959.
[http://dx.doi.org/10.3389/fonc.2020.00959] [PMID: 32766125]
[9]
Zhang, Y.; Zhao, L.; Yang, S.; Cen, Y.; Zhu, T.; Wang, L.; Xia, L.; Liu, Y.; Zou, J.; Xu, J.; Li, Y.; Cheng, X.; Lu, W.; Wang, X.; Xie, X. CircCDKN2B-AS1 interacts with IMP3 to stabilize hexokinase 2 mRNA and facilitate cervical squamous cell carcinoma aerobic glycolysis progression. J. Exp. Clin. Cancer Res., 2020, 39(1), 281.
[http://dx.doi.org/10.1186/s13046-020-01793-7] [PMID: 33308298]
[10]
Chen, L.; Heikkinen, L.; Wang, C.; Yang, Y.; Sun, H.; Wong, G. Trends in the development of miRNA bioinformatics tools. Brief. Bioinform., 2019, 20(5), 1836-1852.
[http://dx.doi.org/10.1093/bib/bby054] [PMID: 29982332]
[11]
Mishra, S.; Yadav, T.; Rani, V. Exploring miRNA based approaches in cancer diagnostics and therapeutics. Crit. Rev. Oncol. Hematol., 2016, 98, 12-23.
[http://dx.doi.org/10.1016/j.critrevonc.2015.10.003] [PMID: 26481951]
[12]
Qu, D.; Yang, Y.; Huang, X. miR-199a-5p promotes proliferation and metastasis and epithelial‐mesenchymal transition through targeting PIAS3 in cervical carcinoma. J. Cell. Biochem., 2019, 120(8), 13562-13572.
[http://dx.doi.org/10.1002/jcb.28631] [PMID: 30937952]
[13]
Zhang, D.; Sun, G.; Peng, L.; Tian, J.; Zhang, H. Calycosin inhibits viability, induces apoptosis, and suppresses invasion of cervical cancer cells by upregulating tumor suppressor miR-375. Arch. Biochem. Biophys., 2020, 691, 108478.
[http://dx.doi.org/10.1016/j.abb.2020.108478]
[14]
Yu, X.; Yang, Y.; Li, Y.; Cao, Y.; Tang, L.; Chen, F.; Xia, J. Baicalein inhibits cervical cancer progression via downregulating long noncoding RNA BDLNR and its downstream PI3K/Akt pathway. Int. J. Biochem. Cell Biol., 2018, 94, 107-118.
[http://dx.doi.org/10.1016/j.abb.2020.108478]
[15]
Bonizzato, A.; Gaffo, E.; te Kronnie, G.; Bortoluzzi, S. CircRNAs in hematopoiesis and hematological malignancies. Blood Cancer J., 2016, 6(10), e483.
[http://dx.doi.org/10.1038/bcj.2016.81] [PMID: 27740630]
[16]
Guarnerio, J.; Bezzi, M.; Jeong, J.C.; Paffenholz, S.V.; Berry, K.; Naldini, M.M.; Lo-Coco, F.; Tay, Y.; Beck, A.H.; Pandolfi, P.P. Oncogenic role of fusion-circRNAs derived from cancer-associated chromosomal translocations. Cell, 2016, 165(2), 289-302.
[http://dx.doi.org/10.1016/j.cell.2016.03.020] [PMID: 27040497]
[17]
Chen, R.X.; Liu, H.L.; Yang, L.L.; Kang, F.H.; Xin, L.P.; Huang, L.R.; Guo, Q.F.; Wang, Y.L. Circular RNA circRNA_0000285 promotes cervical cancer development by regulating FUS. Eur. Rev. Med. Pharmacol. Sci., 2019, 23(20), 8771-8778.
[PMID: 31696463]
[18]
Wang, X.H.; Li, J. CircAGFG1 aggravates the progression of cervical cancer by downregulating p53. Eur. Rev. Med. Pharmacol. Sci., 2020, 24(4), 1704-1711.
[PMID: 32141537]
[19]
Huang, J.; Chen, J.; Huang, Q. The profile analysis of circular RNAs in cervical cancer. Medicine, 2021, 100(39), e27404.
[http://dx.doi.org/10.1097/MD.0000000000027404] [PMID: 34596168]
[20]
Sadri Nahand, J.; Moghoofei, M.; Salmaninejad, A.; Bahmanpour, Z.; Karimzadeh, M.; Nasiri, M.; Mirzaei, H.R.; Pourhanifeh, M.H.; Bokharaei-Salim, F.; Mirzaei, H.; Hamblin, M.R. Pathogenic role of exosomes and microRNAs in HPV-mediated inflammation and cervical cancer: A review. Int. J. Cancer, 2020, 146(2), 305-320.
[http://dx.doi.org/10.1002/ijc.32688] [PMID: 31566705]
[21]
Wei, Y.Q.; Jiao, X.L.; Zhang, S.Y.; Xu, Y.; Li, S.; Kong, B.H. MiR-9-5p could promote angiogenesis and radiosensitivity in cervical cancer by targeting SOCS5. Eur. Rev. Med. Pharmacol. Sci., 2019, 23(17), 7314-7326.
[PMID: 31539118]
[22]
Li, Y.; Ding, Y.; Ding, N.; Zhang, H.; Lu, M.; Cui, X.; Yu, X. MicroRNA-625-5p sponges lncRNA MALAT1 to inhibit cervical carcinoma cell growth by suppressing NF-κB signaling. Cell Biochem. Biophys., 2020, 78(2), 217-225.
[http://dx.doi.org/10.1007/s12013-020-00904-7] [PMID: 32152961]
[23]
Chen, X.; Jiang, J.; Zhao, Y.; Wang, X.; Zhang, C.; Zhuan, L.; Zhang, D.; Zheng, Y. Circular RNA circNTRK2 facilitates the progression of esophageal squamous cell carcinoma through up-regulating NRIP1 expression via miR-140-3p. J. Exp. Clin. Cancer Res., 2020, 39(1), 133.
[http://dx.doi.org/10.1186/s13046-020-01640-9] [PMID: 32653032]
[24]
Tian, Y.; Xu, Z.; Fu, J. CircularRNA-9119 promotes the proliferation of cervical cancer cells by sponging miR-126/MDM4. Mol. Cell. Biochem., 2020, 470(1-2), 53-62.
[http://dx.doi.org/10.1007/s11010-020-03745-3] [PMID: 32385717]
[25]
Wang, Y.; Wang, L.; Wang, W.; Guo, X. Overexpression of circular RNA hsa_circ_0001038 promotes cervical cancer cell progression by acting as a ceRNA for miR-337-3p to regulate cyclin-M3 and metastasis-associated in colon cancer 1 expression. Gene, 2020, 733, 144273.
[http://dx.doi.org/10.1016/j.gene.2019.144273] [PMID: 31809842]
[26]
Jayamohan, S.; Kannan, M.; Moorthy, R.K.; Rajasekaran, N.; Jung, H.S.; Shin, Y.K.; Arockiam, A.J.V. Dysregulation of miR-375/AEG-1 axis by human papillomavirus 16/18-E6/E7 promotes cellular proliferation, migration, and invasion in cervical cancer. Front. Oncol., 2019, 9, 847.
[http://dx.doi.org/10.3389/fonc.2019.00847] [PMID: 31552174]
[27]
Jiang, Y.; Zhao, J.; Li, R.; Liu, Y.; Zhou, L.; Wang, C.; Lv, C.; Gao, L.; Cui, D. CircLRFN5 inhibits the progression of glioblastoma via PRRX2/GCH1 mediated ferroptosis. J. Exp. Clin. Cancer Res., 2022, 41(1), 307.
[http://dx.doi.org/10.1186/s13046-022-02518-8] [PMID: 36266731]
[28]
Mookherjee, T.; Bagchi, A.; Ghosh, R. In silico studies to analyse the possible interactions of CircPPP1R12A translated peptide with Mst proteins. Biochem. Biophys. Res. Commun., 2022, 635, 108-113.
[http://dx.doi.org/10.1016/j.bbrc.2022.10.006] [PMID: 36265283]
[29]
Suresh, P.S.; Tsutsumi, R.; Venkatesh, T. YBX1 at the crossroads of non-coding transcriptome, exosomal, and cytoplasmic granular signaling. Eur. J. Cell Biol., 2018, 97(3), 163-167.
[http://dx.doi.org/10.1016/j.ejcb.2018.02.003] [PMID: 29478751]
[30]
Lasham, A.; Print, C.G.; Woolley, A.G.; Dunn, S.E.; Braithwaite, A.W. YB-1: Oncoprotein, prognostic marker and therapeutic target? Biochem. J., 2013, 449(1), 11-23.
[http://dx.doi.org/10.1042/BJ20121323] [PMID: 23216250]
[31]
Wang, Y.; Su, J.; Wang, Y.; Fu, D.; Ideozu, J.E.; Geng, H.; Cui, Q.; Wang, C.; Chen, R.; Yu, Y.; Niu, Y.; Yue, D. The interaction of YBX1 with G3BP1 promotes renal cell carcinoma cell metastasis via YBX1/G3BP1-SPP1-NF-κB signaling axis. J. Exp. Clin. Cancer Res., 2019, 38(1), 386.
[http://dx.doi.org/10.1186/s13046-019-1347-0] [PMID: 31481087]
[32]
Nagasu, S.; Sudo, T.; Kinugasa, T.; Yomoda, T.; Fujiyoshi, K.; Shigaki, T.; Akagi, Y. Y box binding protein-1 inhibits apoptosis and upregulates EGFR in colon cancer. Oncol. Rep., 2019, 41(5), 2889-2896.
[http://dx.doi.org/10.3892/or.2019.7038] [PMID: 30864697]
[33]
Nishio, S.; Ushijima, K.; Yamaguchi, T.; Sasajima, Y.; Tsuda, H.; Kasamatsu, T.; Kage, M.; Ono, M.; Kuwano, M.; Kamura, T. Nuclear Y-box-binding protein-1 is a poor prognostic marker and related to epidermal growth factor receptor in uterine cervical cancer. Gynecol. Oncol., 2014, 132(3), 703-708.
[http://dx.doi.org/10.1016/j.ygyno.2014.01.045] [PMID: 24486603]
[34]
Pang, T.; Li, M.; Zhang, Y.; Yong, W.; Kang, H.; Yao, Y.; Hu, X. Y-box-binding protein 1 promotes epithelial-mesenchymal transition, invasion, and metastasis of cervical cancer via enhancing the expressions of snail. Int. J. Gynecol. Cancer, 2017, 27(8), 1753-1760.
[http://dx.doi.org/10.1097/IGC.0000000000001066] [PMID: 28708785]
[35]
Dolfini, D.; Mantovani, R. Targeting the Y/CCAAT box in cancer: YB-1 (YBX1) or NF-Y? Cell Death Differ., 2013, 20(5), 676-685.
[http://dx.doi.org/10.1038/cdd.2013.13] [PMID: 23449390]

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy