Crystallization and preliminary x-ray diffraction studies of bnsp-6, a myotoxic phospholipase a2 from bothrops neuwiedi venom

Title:Crystallization and preliminary x-ray diffraction studies of bnsp-6, a myotoxic phospholipase a2 from bothrops neuwiedi venom

Volume: 7 Issue: 6

Author(s): Marcos R. M. Fontes*, Luiz E. Monteiro, Andreimar M. Soares, Veridiana M. Rodrigues, Reinaldo J. da Silva and Jose R. Giglio

Affiliation:

• Departamento de Fisica e Biofisica, Instituto de Biociencias, UNESP, C. P. 510, CEP 18618-000, Botucatu­ SP, Brazil

Abstract: BnSP-6 (myot.oxin I) is a phospholipase A2 homologue isolated from Bothrops neuwiedi pauloensis venom. Crystals of BnSP-6 were obtained which diffracted X-rays to 2.5 A resolution using a synchrotron radiation source at room temperature and belong to space group P3121. The unit cell dimensions are a=b=57.7, c=131.1 A. The structure was solved by molecular replacement using the coordinates of bothropstoxin I from B. jararacussu venom. There are two molecules in the asymmetric unit.

Cite this article as:

Fontes R. M. Marcos*, Monteiro E. Luiz, Soares M. Andreimar, Rodrigues M. Veridiana, da Silva J. Reinaldo and Giglio R. Jose, Crystallization and preliminary x-ray diffraction studies of bnsp-6, a myotoxic phospholipase a2 from bothrops neuwiedi venom, Protein & Peptide Letters 2000; 7 (6) . https://dx.doi.org/10.2174/092986650706221208100629