Isolation and characterization of novel neurotoxins from south american rattlesnake crotalus durissus terrificus: Optimization of chromatographic procedure

Title:Isolation and characterization of novel neurotoxins from south american rattlesnake crotalus durissus terrificus: Optimization of chromatographic procedure

Volume: 7 Issue: 6

Author(s): Marcos Hikari Toyama, Gildo B. Leite, Lea Rodrigues-Simioni, O.S. Saraguacy Hernandez, Jose Camillo. Novello and Sergio Marangoni

Affiliation:

Abstract: Molecular exclusion HPLC chromatography on a Protein Pak SW 300 column and reversed phase HPLC on a µ-Bondapack Cl8 column were used to purify four novel neurotoxins (rom the crotoxin complex, and five crotoxin subunits (PLA2 and crotapotin) as well as two crotamine isoforms. A single preparative reversed phase HPLC steps yielded a major basic myotoxin followed by the subunits of crotoxin (three PLA2 isoforms and two crotapotin isoforms). This modified procedure may be useful for the rapid, large-scale purification of toxins from Crotalid venoms.

Cite this article as:

Toyama Hikari Marcos, Leite B. Gildo, Rodrigues-Simioni Lea, Hernandez Saraguacy O.S., Novello Camillo. Jose and Marangoni Sergio, Isolation and characterization of novel neurotoxins from south american rattlesnake crotalus durissus terrificus: Optimization of chromatographic procedure, Protein & Peptide Letters 2000; 7 (6) . https://dx.doi.org/10.2174/092986650706221207162104