Expression and purification of human metallothionein-3 beta domain in e.coli

Daqing      Wutt; Jiong      Shen; Binggen      Ru

doi:10.2174/092986650704221207123557

Abstract

J3-domain of metallothionein-3/growth inhibitory factor(MT-3/GIF) is crucial to the neuronal growth inhibitory activity of MT-3. The MT-3 J3 domain was efficiently expressed as the soluble fusion form with GST protein at a high level which accounted for over 30% total cellular proteins. After the thrombin cleavage and purification, the bioactive recombinant MT-3 J3 domain was recovered with the yield of 6 mg/L of medium.

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Protein & Peptide Letters

Title:Expression and purification of human metallothionein-3 beta domain in e.coli

Volume: 7 Issue: 4

Author(s): Daqing Wutt, Jiong Shen and Binggen Ru

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Abstract: J3-domain of metallothionein-3/growth inhibitory factor(MT-3/GIF) is crucial to the neuronal growth inhibitory activity of MT-3. The MT-3 J3 domain was efficiently expressed as the soluble fusion form with GST protein at a high level which accounted for over 30% total cellular proteins. After the thrombin cleavage and purification, the bioactive recombinant MT-3 J3 domain was recovered with the yield of 6 mg/L of medium.

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Wutt Daqing, Shen Jiong and Ru Binggen, Expression and purification of human metallothionein-3 beta domain in e.coli, Protein & Peptide Letters 2000; 7 (4) . https://dx.doi.org/10.2174/092986650704221207123557

DOI https://dx.doi.org/10.2174/092986650704221207123557	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305