Further characterization of the glycan-binding specificity of the seed lectin from vatairea macrocarpa and its dependence of pH

Title:Further characterization of the glycan-binding specificity of the seed lectin from vatairea macrocarpa and its dependence of pH

Volume: 7 Issue: 4

Author(s): Marcio V. Ramos*, Liezelotte R. Bomfim, Benildo S. Cavada, Nylane M.N. Alencar, Thalles B. Grangeiro and Henri Debray

Affiliation:

• Departamentos de Biologia, Caixa Postal 6033. 60.451-970 Fortaleza-Ceara, Brasil

Abstract: The lectin from Vatairea macrocarpa seeds exhibits a pronounced specificity for glycoproteins possessing Gal/GalNAc residues in their carbohydrate moieties. Determination of the fine sugar specificity of the lectin by affinity chromatography of labelled oligosaccharides and glycopeptides of known structures on the immobilized lectin shows that the lectin precisely recognizes non reducing N acetyllactosamine sequence. This interaction seems to depend of the number of N-acetyllactosamine sequence in each structure and is dependent of pH.

Cite this article as:

Ramos V. Marcio*, Bomfim R. Liezelotte, Cavada S. Benildo, Alencar M.N. Nylane, Grangeiro B. Thalles and Debray Henri, Further characterization of the glycan-binding specificity of the seed lectin from vatairea macrocarpa and its dependence of pH, Protein & Peptide Letters 2000; 7 (4) . https://dx.doi.org/10.2174/092986650704221206164217