Sensitive fluorogenic substrates for plasmepsin 2

Title:Sensitive fluorogenic substrates for plasmepsin 2

Volume: 7 Issue: 4

Author(s): Sergei V. Gulnik*, Leonid I. Suvorov, Pavel Majer and John W. Erickson

Affiliation:

• Structural Biochemistry Program, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, P.O Box B, Frederick, MD 21702-1201

Abstract: Structural Biochemistry Program, SAIC Frederick, National Cancer Institute-Frederick Cancer Research and Development Center, P.O Box B, Frederick, MD 21702-1201. Present Address: Tibotec Inc., 1330 Piccard Dr., Rockville, MD, 20850. 1Present Address: Gilford Pharmaceuticals Inc., 6611 Tributary Street, Baltimore, MD, 21224. Plasmepsin 2 is a malarial aspartic proteinase that has been implicated in initial steps of hemoglobin degradation in parasite and thus represents an attractive antimalarial target. We designed and synthesized a number of quenched fluorogenic substrates and measured the kinetic constants of their cleavage by plasmepsin-2. High sensitivity and superior kinetic properties make the substrates suitable for different application including fluorescence plate reader.

Cite this article as:

Gulnik V. Sergei*, Suvorov I. Leonid, Majer Pavel and Erickson W. John, Sensitive fluorogenic substrates for plasmepsin 2, Protein & Peptide Letters 2000; 7 (4) . https://dx.doi.org/10.2174/092986650704221206162203