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Current Molecular Medicine

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ISSN (Print): 1566-5240
ISSN (Online): 1875-5666

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Short Communication

Direct SARS-CoV-2 Detection System Utilizing Simple-to-Use Capillary Gel Electrophoresis Sample-to-Result

Author(s): Neo Yang, Meya Kuo, Jerry Liu, Varoujan Amirkhanian* and Eric Tsai

Volume 24, Issue 1, 2024

Published on: 30 December, 2022

Page: [145 - 150] Pages: 6

DOI: 10.2174/1566524023666221104160148

Price: $65

Abstract

Aim: We present a Direct SARS-CoV-2 Detection System that achieves sample-to-results in less than two hours in three simple steps.

Methods: The Detection System includes Direct one-step Reverse Transcription PCR (RT-PCR) reagents (Qexp-MDx kit), a portable thermal cycler (Qampmini) with a preprogrammed chip and a simple-to-use Capillary Gel Electrophoresis system (Qsep Series Bio-Fragment Analyzer) with high fluorescence detection sensitivity to solve the problems associated with traditional real-time PCR (qPCR) systems which produces high false positive results.

Results: The proposed simple-to-use detection platform can provide high detection sensitivity (identify less than 20 copies) and fast results (less than 120 minutes), which would be suitable for field testing applications.

Conclusion: Our high detection sensitivity platform provides fast and accurate results in 120 minutes without doing DNA/RNA extraction.

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